PMID- 10350638
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20201209
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 69
IP  - 1
DP  - 1999 May 21
TI  - Genomic organization and regulatory elements of the rat latexin gene, which is
      expressed in a cell type-specific manner in both central and peripheral nervous
      systems.
PG  - 62-72
AB  - Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific
      manner in both central and peripheral nervous systems in the rat. In the
      neocortex, a specific subpopulation of neurons in layers V and VI expresses
      latexin. In the primary sensory ganglia, the expression is restricted to smaller 
      diameter neurons. As a first step to clarify regulatory mechanisms underlying
      cell type-specific expression of latexin, we have determined the organization of 
      the rat latexin gene and analyzed its regulatory elements. The latexin gene spans
      approximately 5.8 kb, and consists of six exons and five introns. Three
      transcription initiation sites were mapped. The upstream region lacks typical
      TATA or CAAT boxes but has several GC-rich sites. To assess promoter activity,
      the luciferase reporter gene fused to the 5'-flanking region (6.4 kb) of the
      latexin gene was transiently transfected into several cell lines. Luciferase
      activity was 2-8 times higher in latexin-expressing cells (PC12) than
      non-expressing cells (NS20 and L6). Deletion analysis with PC12 cells revealed
      that a core promoter is located between nucleotide positions -261 and -201
      relative to the A of the initiation codon. Nerve growth factor (NGF)-responsive
      element(s) is located between positions -518 and -262, in which AP-1, AP-2 and
      NF-kappaB binding sites are found. Furthermore, we demonstrate that a 1.3 kb
      genomic fragment containing the first intron has transcriptional enhancing
      activity in PC12 cells. These results suggest that up and downstream regulatory
      elements are involved in the control of cell type-specific expression of latexin.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Miyasaka, N
AU  - Miyasaka N
AD  - Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo
      194-8511, Japan. miyasaka@libra.ls.m-kagaku.co.jp
FAU - Hatanaka, Y
AU  - Hatanaka Y
FAU - Jin, M
AU  - Jin M
FAU - Arimatsu, Y
AU  - Arimatsu Y
LA  - eng
SI  - GENBANK/X76985
SI  - GENBANK/Y18435
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Lxn protein, rat)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.4.- (Carboxypeptidases)
RN  - EC 3.4.17.1 (Carboxypeptidases A)
SB  - IM
MH  - 5' Untranslated Regions/physiology
MH  - Animals
MH  - Antigens/*genetics
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Carboxypeptidases/analysis
MH  - Carboxypeptidases A
MH  - Cerebral Cortex/chemistry/cytology
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Enhancer Elements, Genetic/physiology
MH  - Ganglia, Sensory/chemistry/cytology
MH  - Gene Expression Regulation/physiology
MH  - Genes, Reporter
MH  - Genome
MH  - Introns/genetics
MH  - Luciferases/genetics
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurons, Afferent/*chemistry/enzymology
MH  - PC12 Cells
MH  - Plasmids
MH  - Promoter Regions, Genetic/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transfection
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0169328X99001072 [pii]
AID - 10.1016/s0169-328x(99)00107-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 May 21;69(1):62-72. doi:
      10.1016/s0169-328x(99)00107-2.