PMID- 10350628
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1431
IP  - 2
DP  - 1999 May 18
TI  - Cloning and preliminary characterization of a 121 kDa protein with multiple
      predicted C2 domains.
PG  - 525-30
AB  - In the course of characterizing proteins present in a preparation of vesicles
      from rat adipocytes containing glucose transporters, we examined a protein that
      migrated at 115 kDa upon SDS gel electrophoresis (designated vp115). Sequences of
      tryptic peptides were obtained, and from this information the cDNA for rat vp115 
      was cloned. The cDNA encodes an open reading frame for a protein of 121 kDa.
      Computer-aided sequence analysis predicted that vp115 has a potential
      membrane-inserted or membrane-spanning domain toward its amino terminus, followed
      by five C2 domains. Immunoblotting revealed that vp115 was not actually a
      component of the glucose transporter-containing vesicles, was most abundant in
      the plasma membranes and high density microsome fractions of rat adipocytes, and 
      was expressed in all the major rat tissues.
FAU - Morris, N J
AU  - Morris NJ
AD  - Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
FAU - Ross, S A
AU  - Ross SA
FAU - Neveu, J M
AU  - Neveu JM
FAU - Lane, W S
AU  - Lane WS
FAU - Lienhard, G E
AU  - Lienhard GE
LA  - eng
SI  - GENBANK/AF098633
SI  - GENBANK/AF098634
SI  - GENBANK/AF099138
GR  - DK09401/DK/NIDDK NIH HHS/United States
GR  - DK25336/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Membrane Proteins)
RN  - 0 (vp115 protein, rat)
SB  - IM
MH  - Adipocytes/chemistry/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Immunoblotting
MH  - Membrane Proteins/analysis/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sequence Homology
MH  - Subcellular Fractions/metabolism
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0167-4838(99)00068-0 [pii]
AID - 10.1016/s0167-4838(99)00068-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 May 18;1431(2):525-30. doi:
      10.1016/s0167-4838(99)00068-0.