PMID- 10350130
OWN - NLM
STAT- MEDLINE
DCOM- 19990713
LR  - 20190905
IS  - 0340-2061 (Print)
IS  - 0340-2061 (Linking)
VI  - 199
IP  - 6
DP  - 1999 Jun
TI  - Trefoil factor family (TFF)-domain peptides in the mouse: embryonic
      gastrointestinal expression and wounding response.
PG  - 499-508
AB  - UNLABELLED: Trefoil factor family (TFF)-domain peptides are mucin-associated
      molecules that play a role in maintaining gastrointestinal (GI) epithelial
      integrity. They are expressed in specific patterns in adult mammals, but their
      embryonic expression has not been clarified. Developmental TFF mRNA in mice was
      studied by non-isotopic whole mount in situ hybridization. All TFF's (1-3) were
      seen in the stomach from E13 to E16. TFF1 was gastric at E13, then spread to the 
      small intestine (E15) and caecum on E16. Froin E19 TFF1 expression was gastric.
      TFF2 was gastric at E13, and absent in lower intestines till E17 when duodenal,
      small intestinal and caecal expression was seen. Afterwards, TFF2 was confined to
      the gastric region. TFF3 was in the stomach at E13. On E15 and 16 TFF3 was
      ubiquitous, except for E15 caecum. From E17, TFF3 was confined to small intestine
      and the distal gut. WOUNDS: E17 and 18 GI tissues were subjected to incisional
      wounds in vitro. TFF1 induction was seen only in stomach, after as short as 30
      min incubation. TFF2 was only induced at E18 in the stomach. TFF3 was induced
      within 5 min in the rectum. No change in overall expression patterns were seen
      after wounding. CONCLUSIONS: TFF expression is developmentally controlled in the 
      GI tract, and appears before mucous cell differentiation in several tissues. Gene
      regulation is predicted to be under different control(s) in utero compared with
      post-natal life. The response to incisional wounding of fetal GI tissue shows
      differences to the adult.
FAU - Otto, W R
AU  - Otto WR
AD  - Histopathology Unit, Imperial Cancer Research Fund, London, UK.
      w.otto@icrf.icnet.uk
FAU - Patel, K
AU  - Patel K
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Anat Embryol (Berl)
JT  - Anatomy and embryology
JID - 7505194
RN  - 0 (Growth Substances)
RN  - 0 (Mucins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (TFF3 protein, rat)
RN  - 0 (Tff2 protein, rat)
RN  - 0 (Trefoil Factor-2)
RN  - 0 (Trefoil Factor-3)
SB  - IM
MH  - Animals
MH  - Digestive System/*embryology/metabolism
MH  - Embryo, Mammalian/physiology
MH  - Embryonic and Fetal Development/physiology
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Growth Substances/*genetics/metabolism
MH  - In Situ Hybridization
MH  - Intestinal Mucosa/cytology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Mucins
MH  - *Muscle Proteins
MH  - *Neuropeptides
MH  - Peptides/*genetics/metabolism
MH  - Pregnancy
MH  - *Prenatal Injuries
MH  - RNA, Messenger/biosynthesis
MH  - Trefoil Factor-2
MH  - Trefoil Factor-3
MH  - Wound Healing/*physiology
EDAT- 1999/06/01 00:00
MHDA- 1999/06/01 00:01
CRDT- 1999/06/01 00:00
PHST- 1999/06/01 00:00 [pubmed]
PHST- 1999/06/01 00:01 [medline]
PHST- 1999/06/01 00:00 [entrez]
AID - 10.1007/s004290050247 [doi]
PST - ppublish
SO  - Anat Embryol (Berl). 1999 Jun;199(6):499-508. doi: 10.1007/s004290050247.