PMID- 10348902
OWN - NLM
STAT- MEDLINE
DCOM- 19991108
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 6
DP  - 1999 Jun
TI  - Molecular cloning of Ca2+/calmodulin-dependent protein kinase phosphatase.
PG  - 1022-8
AB  - Calmodulin-dependent protein kinase (CaM-kinase) phosphatase dephosphorylates and
      concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and
      CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase [Ishida,
      I., Okuno, S., Kitani, T., Kameshita, I., and Fujisawa, H. (1998) Biochem.
      Biophys. Res. Commun. 253, 159-163], suggesting that CaM-kinase phosphatase plays
      important roles in the function of Ca2+ in the cell, because the three
      multifunctional CaM-kinases (CaM-kinases I, II, and IV) are thought to be the key
      enzymes in the Ca2+-signaling system. In the present study, cDNA for CaM-kinase
      phosphatase was cloned from a rat brain cDNA library. The coded protein consisted
      of 450 amino acids with a molecular weight of 49, 165. Western blot analysis
      showed the ubiquitous tissue distribution of CaM-kinase phosphatase.
      Immunocytochemical analysis revealed that CaM-kinase phosphatase is evenly
      distributed outside the nucleus in a cell.
FAU - Kitani, T
AU  - Kitani T
AD  - Department of Biochemistry, Asahikawa Medical College, Asahikawa, Hokkaido
      078-8510, Japan.
FAU - Ishida, A
AU  - Ishida A
FAU - Okuno, S
AU  - Okuno S
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Kameshita, I
AU  - Kameshita I
FAU - Fujisawa, H
AU  - Fujisawa H
LA  - eng
SI  - GENBANK/AB023634
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/enzymology
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Escherichia coli/genetics
MH  - Humans
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Protein Tyrosine Phosphatases/chemistry/*genetics/metabolism
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Subcellular Fractions/enzymology
MH  - Tissue Distribution
EDAT- 1999/06/01 00:00
MHDA- 1999/06/01 00:01
CRDT- 1999/06/01 00:00
PHST- 1999/06/01 00:00 [pubmed]
PHST- 1999/06/01 00:01 [medline]
PHST- 1999/06/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022381 [doi]
PST - ppublish
SO  - J Biochem. 1999 Jun;125(6):1022-8. doi: 10.1093/oxfordjournals.jbchem.a022381.