PMID- 10348810
OWN - NLM
STAT- MEDLINE
DCOM- 19990630
LR  - 20190707
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 116
IP  - 6
DP  - 1999 Jun
TI  - Urocortin reduces food intake and gastric emptying in lean and ob/ob obese mice.
PG  - 1287-92
AB  - BACKGROUND & AIMS: Gastric emptying plays an important role in regulating food
      intake. This study was designed to investigate whether intraperitoneally injected
      urocortin reduces gastric emptying, feeding, and body weight in lean and ob/ob
      obese mice. METHODS: Food intake and body weight were measured after
      intraperitoneal injections of one of the following: urocortin, deamidated form of
      urocortin (urocortin OH), corticotropin-releasing factor (CRF), CRF6-33,
      cholecystokinin octapeptide (CCK-8), and leptin in 16-hour food-deprived animals.
      Gastric emptying was assessed 2, 4, or 8 hours after intraperitoneal injection.
      Repeated injections of urocortin were continued for 5 days in ob/ob mice.
      RESULTS: Urocortin (0.003-3 nmol) dose-dependently and potently decreased food
      intake and body weight gain in lean mice. The ranking order of potency was
      urocortin > urocortin OH >/= CRF > CCK-8 > CRF6-33 > leptin. Gastric emptying was
      also potently reduced by urocortin with a similar ranking order of potency of
      urocortin > CRF > urocortin OH > CCK-8. Simultaneous administration of urocortin 
      and CRF receptor antagonist, alpha-helical CRF9-41, blocked the effects of
      urocortin. Urocortin reduced food intake and body weight gain, as well as the
      rate of gastric emptying, in ob/ob mice, which was significantly faster than that
      of lean mice. Five daily injections of urocortin significantly lowered body
      weight and improved glycemic control in ob/ob mice. CONCLUSIONS: The
      urocortin-induced decrease in food intake and body weight in lean and ob/ob mice 
      is closely related to gastric emptying and opens new possibilities for the
      treatment of obesity.
FAU - Asakawa, A
AU  - Asakawa A
AD  - Second Department of Internal Medicine, Kobe University School of Medicine, Kobe,
      Japan.
FAU - Inui, A
AU  - Inui A
FAU - Ueno, N
AU  - Ueno N
FAU - Makino, S
AU  - Makino S
FAU - Fujino, M A
AU  - Fujino MA
FAU - Kasuga, M
AU  - Kasuga M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Blood Glucose)
RN  - 0 (Leptin)
RN  - 0 (Proteins)
RN  - 0 (Urocortins)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - M03GIQ7Z6P (Sincalide)
SB  - AIM
SB  - IM
CIN - Gastroenterology. 1999 Jun;116(6):1487-9. PMID: 10348833
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Body Weight/drug effects
MH  - Corticotropin-Releasing Hormone/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Eating/*drug effects
MH  - Gastric Emptying/*drug effects
MH  - Injections, Intraperitoneal
MH  - Leptin
MH  - Male
MH  - Mice/genetics
MH  - Obesity/genetics/*physiopathology
MH  - Proteins/pharmacology
MH  - Reference Values
MH  - Sincalide/pharmacology
MH  - Urocortins
EDAT- 1999/05/29 00:00
MHDA- 1999/05/29 00:01
CRDT- 1999/05/29 00:00
PHST- 1999/05/29 00:00 [pubmed]
PHST- 1999/05/29 00:01 [medline]
PHST- 1999/05/29 00:00 [entrez]
AID - S0016-5085(99)70491-9 [pii]
AID - 10.1016/s0016-5085(99)70491-9 [doi]
PST - ppublish
SO  - Gastroenterology. 1999 Jun;116(6):1287-92. doi: 10.1016/s0016-5085(99)70491-9.