PMID- 10347222
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 23
DP  - 1999 Jun 4
TI  - Cloning, tissue distribution, genomic organization, and functional
      characterization of NBC3, a new member of the sodium bicarbonate cotransporter
      family.
PG  - 16569-75
AB  - Previous functional studies have demonstrated that muscle intracellular pH
      regulation is mediated by sodium-coupled bicarbonate transport, Na+/H+ exchange, 
      and Cl-/bicarbonate exchange. We report the cloning, sequence analysis, tissue
      distribution, genomic organization, and functional analysis of a new member of
      the sodium bicarbonate cotransporter (NBC) family, NBC3, from human skeletal
      muscle. mNBC3 encodes a 1214-residue polypeptide with 12 putative
      membrane-spanning domains. The approximately 7.8-kilobase transcript is expressed
      uniquely in skeletal muscle and heart. The NBC3 gene (SLC4A7) spans approximately
      80 kb and is composed of 25 coding exons and 24 introns that are flanked by
      typical splice donor and acceptor sequences. Expression of mNBC3 cRNA in Xenopus 
      laevis oocytes demonstrated that the protein encodes a novel stilbene-insensitive
      5-(N-ethyl-N-isopropyl)-amiloride-inhibitable sodium bicarbonate cotransporter.
FAU - Pushkin, A
AU  - Pushkin A
AD  - Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los 
      Angeles, California 90095-1698, USA.
FAU - Abuladze, N
AU  - Abuladze N
FAU - Lee, I
AU  - Lee I
FAU - Newman, D
AU  - Newman D
FAU - Hwang, J
AU  - Hwang J
FAU - Kurtz, I
AU  - Kurtz I
LA  - eng
SI  - GENBANK/AF047033
GR  - DK46976/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (SLC4A7 protein, human)
RN  - 0 (Sodium-Bicarbonate Symporters)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Expressed Sequence Tags
MH  - Humans
MH  - Membrane Proteins/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Oocytes/chemistry/metabolism
MH  - Protein Structure, Secondary
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sodium-Bicarbonate Symporters
MH  - Xenopus laevis
EDAT- 1999/05/29 00:00
MHDA- 1999/05/29 00:01
CRDT- 1999/05/29 00:00
PHST- 1999/05/29 00:00 [pubmed]
PHST- 1999/05/29 00:01 [medline]
PHST- 1999/05/29 00:00 [entrez]
AID - 10.1074/jbc.274.23.16569 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 4;274(23):16569-75. doi: 10.1074/jbc.274.23.16569.