PMID- 10347202
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 23
DP  - 1999 Jun 4
TI  - Two novel Kruppel-associated box-containing zinc-finger proteins, KRAZ1 and
      KRAZ2, repress transcription through functional interaction with the corepressor 
      KAP-1 (TIF1beta/KRIP-1).
PG  - 16412-22
AB  - We have isolated two novel Kruppel-like zinc finger proteins containing the
      evolutionarily conserved Kruppel-associated box (KRAB), KRAZ1 and KRAZ2, and
      demonstrated that they repress transcription when heterologously targeted to DNA.
      Their repression activity appeared to be mediated by the putative corepressor
      KAP-1 (KRAB-associated protein-1), because KRAZ1/2 bind to KAP-1, but KRAB
      mutants of KRAZ1/2 that are unable to interact with KAP-1 lack repression
      activity, and KAP-1 has intrinsic repressor activity and potentiates
      KRAZ1/2-mediated repression. We dissected the KAP-1 protein into a
      KRAB-interacting domain and a region necessary for repression. Using a mammalian 
      two-hybrid assay, we further demonstrated that KAP-1 deletions lacking repression
      activity fused to the VP16 transactivation domain strongly activated
      transcription when coexpressed with KRAZ1. In contrast, VP16-KAP-1 fusions
      retaining repression activity resulted in repression. These results provide the
      first evidence that KAP-1 functionally interacts with KRAB in mammalian cells and
      seems to exert repressor activity in the DNA-bound KRAB-KAP-1 complex, and they
      further support the hypothesis that KAP-1 functions as a corepressor for the
      large class of KRAB-containing zinc finger proteins.
FAU - Agata, Y
AU  - Agata Y
AD  - Center for Molecular Biology and Genetics, Kyoto University, Sakyo-ku, Kyoto
      606-8501, Japan.
FAU - Matsuda, E
AU  - Matsuda E
FAU - Shimizu, A
AU  - Shimizu A
LA  - eng
SI  - GENBANK/AB024004
SI  - GENBANK/AB024005
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZNF10 protein, human)
RN  - 0 (Zfp118 protein, mouse)
RN  - 0 (Zfp68 protein, mouse)
RN  - EC 2.3.2.27 (Trim28 protein, mouse)
RN  - EC 2.3.2.27 (Tripartite Motif-Containing Protein 28)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - DNA-Binding Proteins/*metabolism
MH  - Kruppel-Like Transcription Factors
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Peptide Mapping
MH  - Promoter Regions, Genetic
MH  - Repressor Proteins/isolation & purification/*metabolism
MH  - *Transcription Factors
MH  - Transcription, Genetic
MH  - Tripartite Motif-Containing Protein 28
MH  - *Zinc Fingers
EDAT- 1999/05/29 00:00
MHDA- 1999/05/29 00:01
CRDT- 1999/05/29 00:00
PHST- 1999/05/29 00:00 [pubmed]
PHST- 1999/05/29 00:01 [medline]
PHST- 1999/05/29 00:00 [entrez]
AID - 10.1074/jbc.274.23.16412 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 4;274(23):16412-22. doi: 10.1074/jbc.274.23.16412.