PMID- 10344264
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 20
IP  - 4-5
DP  - 1999 Apr-May
TI  - A two-dimensional electrophoresis database of rat heart proteins.
PG  - 891-7
AB  - More than 3000 myocardial protein species of Wistar Kyoto rat, an important
      animal model, were separated by high-resolution two-dimensional gel
      electrophoresis (2-DE) and characterized in terms of isoelectric point (pI) and
      molecular mass (Mr). Currently, the 2-DE database contains 64 identified
      proteins; forty-three were identified by peptide mass fingerprinting (PMF) using 
      matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), nine by
      exclusive comparison with other 2-DE heart protein databases, and in only 12
      cases of 60 attempts N-terminal sequencing was successful. We used the Make2ddb
      software package downloaded from the ExPASy server for the construction of a rat 
      myocardial 2-DE database. The Make2ddb package simplifies the creation of a new
      2-DE database if the Melanie II software and a Sun workstation under Solaris are 
      available. Our 2-DE database of rat heart proteins can be accessed at URL
      http://gelmatching.inf.fu-berlin.de/pleiss/2d.
FAU - Li, X P
AU  - Li XP
AD  - Max Volmer Institute of Biophysical Chemistry and Biochemistry, Technical
      University Berlin, Germany.
FAU - Pleissner, K P
AU  - Pleissner KP
FAU - Scheler, C
AU  - Scheler C
FAU - Regitz-Zagrosek, V
AU  - Regitz-Zagrosek V
FAU - Salnikow, J
AU  - Salnikow J
FAU - Jungblut, P R
AU  - Jungblut PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - *Databases, Factual
MH  - Electrophoresis, Gel, Two-Dimensional/*methods
MH  - Heart
MH  - Myocardium/*chemistry
MH  - Proteins/*analysis
MH  - Rats
MH  - Rats, Inbred WKY
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 1999/05/27 06:00
MHDA- 2000/08/12 11:00
CRDT- 1999/05/27 06:00
PHST- 1999/05/27 06:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/05/27 06:00 [entrez]
AID - 10.1002/(SICI)1522-2683(19990101)20:4/5<891::AID-ELPS891>3.0.CO;2-2 [pii]
AID - 10.1002/(SICI)1522-2683(19990101)20:4/5<891::AID-ELPS891>3.0.CO;2-2 [doi]
PST - ppublish
SO  - Electrophoresis. 1999 Apr-May;20(4-5):891-7. doi:
      10.1002/(SICI)1522-2683(19990101)20:4/5<891::AID-ELPS891>3.0.CO;2-2.