PMID- 10343284
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20191024
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 22
IP  - 3
DP  - 1999 Jun
TI  - Gene and cDNA cloning and characterization of the mouse V3/V1b pituitary
      vasopressin receptor.
PG  - 251-60
AB  - The gene of the mouse V3/V1b receptor was identified by homology cloning. One of 
      the genomic clones contained the entire coding sequence. The cDNA presented high 
      identity with rat (92%) and human (84%) sequences. Southern blot analysis
      indicated the existence of a single gene. Tissue distribution was studied by
      RT-PCR. The major site of expression was the pituitary. A faint signal was also
      present in hypothalamus, brain, adrenal, pancreas and colon. The mouse
      corticotroph cell line, AtT20, did not express the transcript. In order to
      confirm the identity of the sequence, the V3/V1b receptor cDNA was cloned and
      stably expressed in CHO-AA8 Tet-Off cells under the control of tetracycline. When
      transfected cells were treated with arginine vasopressin (AVP), inositol
      phosphate production increased in a dose-dependent manner, indicating that the
      V3/V1b receptor couples to phospholipase C. Moreover, AVP did not stimulate cAMP 
      production. Binding studies with [3H]AVP indicated that the affinity of the mouse
      V3/V1b receptor (Kd=0.5 nM) is similar to that reported for rat and human
      receptors. The rank order of potency established in competition binding
      experiments with different analogues was representative of a V3/V1b profile,
      distinct from V1a and V2. However, significant differences were found between
      human and mouse receptors tested in parallel. Thus the pharmacology of V3/V1b
      receptors can not be transposed among different species.
FAU - Ventura, M A
AU  - Ventura MA
AD  - Groupe d'Etudes en Physiopathologie Endocrinienne, UPR 1524 CNRS, Institut Cochin
      de Genetique Moleculaire, CHU Cochin, Universite Paris V, 75014 Paris.
FAU - Rene, P
AU  - Rene P
FAU - de Keyzer, Y
AU  - de Keyzer Y
FAU - Bertagna, X
AU  - Bertagna X
FAU - Clauser, E
AU  - Clauser E
LA  - eng
SI  - GENBANK/AF098866
SI  - GENBANK/AF098867
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Codon)
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Vasopressin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Codon
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Pituitary Gland/*metabolism
MH  - Protein Biosynthesis
MH  - Radioligand Assay
MH  - Receptors, Vasopressin/drug effects/*genetics/physiology
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/27 00:00
MHDA- 1999/05/27 00:01
CRDT- 1999/05/27 00:00
PHST- 1999/05/27 00:00 [pubmed]
PHST- 1999/05/27 00:01 [medline]
PHST- 1999/05/27 00:00 [entrez]
AID - JME00783 [pii]
AID - 10.1677/jme.0.0220251 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 1999 Jun;22(3):251-60. doi: 10.1677/jme.0.0220251.