PMID- 10343118
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 84
IP  - 1-2
DP  - 1999
TI  - cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9),
      encoding an N-terminally extended human ubiquitin-conjugating enzyme.
PG  - 99-104
AB  - A cDNA encoding a third member of human class III ubiquitin-conjugating enzymes
      (E2s), UBE2E3, was cloned from a human gastric adenocarcinoma cDNA library. The
      deduced 207-amino acid protein shares over 94% amino acid identity with the UBC
      domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the
      N-terminal extension exhibited little homology among these, except for UbcM2,
      which showed 100% identity, and which is thought to be a mouse counterpart.
      Northern hybridization analysis exhibited a strong 1.9-kb band of UBE2E3 in
      skeletal muscle. Recombinant fusion protein of GST-UBE2E3 was found to form a
      thioester bond with ubiquitin (Ub) in an E1-dependent manner, demonstrating that 
      the cDNA encodes a functional E2. In addition, a UBE2E3 mutant of cysteine-145 to
      serine failed in UBE2E3-Ub complex formation, indicating that the cysteine is
      essential for E2 function. Using FISH and PCR analysis of radiation hybrid and
      somatic cell hybrid panels the UBE2E3 gene was mapped to human chromosome 2q32.1 
      and showed strong linkage to SHGC-8506 (LOD = 11.52) between D2S1302 and D2S364.
FAU - Ito, K
AU  - Ito K
AD  - Department of Molecular Pathobiochemistry, Gifu University School of Medicine,
      Gifu, Japan.
FAU - Kato, S
AU  - Kato S
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Kimura, M
AU  - Kimura M
FAU - Okano, Y
AU  - Okano Y
LA  - eng
SI  - GENBANK/AB017644
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.3.2.23 (UBE2E2 protein, human)
RN  - EC 2.3.2.23 (UBE2E3 protein, human)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/*genetics
MH  - Genetic Linkage
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Ubiquitin-Conjugating Enzymes/*genetics
EDAT- 1999/05/27 06:00
MHDA- 2000/08/16 11:00
CRDT- 1999/05/27 06:00
PHST- 1999/05/27 06:00 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1999/05/27 06:00 [entrez]
AID - 15229 [pii]
AID - 10.1159/000015229 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1999;84(1-2):99-104. doi: 10.1159/000015229.