PMID- 10342878
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20151119
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 140
IP  - 6
DP  - 1999 Jun
TI  - Differential expression of a novel seven transmembrane domain protein in
      epididymal fat from aged and diabetic mice.
PG  - 2859-67
AB  - To identify novel seven transmembrane domain proteins from 3T3-L1 adipocytes, we 
      used PCR to amplify 3T3-L1 adipocyte complementary DNA (cDNA) with primers
      homologous to the N- and C-termini of pancreatic glucagon-like peptide-1 (GLP-1) 
      receptor. We screened a cDNA library prepared from fully differentiated 3T3-L1
      adipocytes using a 500-bp cDNA PCR product probe. Herein describes the isolation 
      and characterization of a 1.6-kb cDNA clone that encodes a novel 298-amino acid
      protein that we termed TPRA40 (transmembrane domain protein of 40 kDa regulated
      in adipocytes). TPRA40 has seven putative transmembrane domains and shows little 
      homology with the known GLP-1 receptor or with other G protein-coupled receptors.
      The levels of TPRA40 mRNA and protein were higher in 3T3-L1 adipocytes than in
      3T3-L1 fibroblasts. TPRA40 is present in a number of mouse and human tissues.
      Interestingly, TPRA40 mRNA levels were significantly increased by 2- to 3-fold in
      epididymal fat of 24-month-old mice vs. young controls as well as in db/db and
      ob/ob mice vs. nondiabetic control littermates. No difference in TPRA40 mRNA
      levels was observed in brain, heart, skeletal muscle, liver, or kidney.
      Furthermore, no difference in TPRA40 expression was detected in brown fat of
      ob/ob mice when compared with age-matched controls. Taken together, these data
      suggest that TPRA40 represents a novel membrane-associated protein whose
      expression in white adipose tissue is altered with aging and type 2 diabetes.
FAU - Yang, H
AU  - Yang H
AD  - Diabetes Section, Laboratory of Clinical Investigation, National Institute on
      Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.
FAU - Egan, J M
AU  - Egan JM
FAU - Rodgers, B D
AU  - Rodgers BD
FAU - Bernier, M
AU  - Bernier M
FAU - Montrose-Rafizadeh, C
AU  - Montrose-Rafizadeh C
LA  - eng
SI  - GENBANK/AF051098
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (DNA, Complementary)
RN  - 0 (GLP1R protein, human)
RN  - 0 (Glp1r protein, mouse)
RN  - 0 (Glucagon-Like Peptide-1 Receptor)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Glucagon)
SB  - AIM
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*chemistry
MH  - Aging/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/isolation & purification
MH  - Diabetes Mellitus/*metabolism
MH  - Epididymis/*chemistry
MH  - Glucagon-Like Peptide-1 Receptor
MH  - Male
MH  - Membrane Proteins/*analysis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese/metabolism
MH  - Molecular Sequence Data
MH  - Protein Isoforms/analysis
MH  - Receptors, Glucagon/*analysis/genetics
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1210/endo.140.6.6830 [doi]
PST - ppublish
SO  - Endocrinology. 1999 Jun;140(6):2859-67. doi: 10.1210/endo.140.6.6830.