PMID- 10341243
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 11
DP  - 1999 Jun 1
TI  - Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal 
      differentiation.
PG  - 4421-7
AB  - Alzheimer's amyloid precursor protein (APP), the precursor of beta-amyloid
      (Abeta), is an integral membrane protein with a receptor-like structure. We
      recently demonstrated that the mature APP (mAPP; N- and O-glycosylated form) is
      phosphorylated at Thr668 (numbering for APP695 isoform), specifically in neurons.
      Phosphorylation of mAPP appears to occur during, and after, neuronal
      differentiation. Here we report that the phosphorylation of mAPP begins 48-72 hr 
      after treatment of PC12 cells with NGF and that this correlates with the timing
      of neurite outgrowth. The phosphorylated form of APP is distributed in neurites
      and mostly in the growth cones of differentiating PC12 cells. PC12 cells stably
      expressing APP with Thr668Glu substitution showed remarkably reduced neurite
      extension after treatment with NGF. These observations suggest that the
      phosphorylated form of APP may play an important role in neurite outgrowth of
      differentiating neurons.
FAU - Ando, K
AU  - Ando K
AD  - Laboratory of Neurobiophysics, School of Pharmaceutical Sciences, The University 
      of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033 Japan.
FAU - Oishi, M
AU  - Oishi M
FAU - Takeda, S
AU  - Takeda S
FAU - Iijima, K
AU  - Iijima K
FAU - Isohara, T
AU  - Isohara T
FAU - Nairn, A C
AU  - Nairn AC
FAU - Kirino, Y
AU  - Kirino Y
FAU - Greengard, P
AU  - Greengard P
FAU - Suzuki, T
AU  - Suzuki T
LA  - eng
GR  - P01 AG009464/AG/NIA NIH HHS/United States
GR  - AG09464/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 2ZD004190S (Threonine)
SB  - IM
MH  - Alzheimer Disease/*metabolism/pathology
MH  - Amyloid beta-Protein Precursor/genetics/*physiology
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Mutation
MH  - Nerve Tissue Proteins/genetics/*physiology
MH  - Neurons/*metabolism/pathology
MH  - PC12 Cells
MH  - Phosphorylation
MH  - Rats
MH  - Threonine/physiology
PMC - PMC6782598
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Jun 1;19(11):4421-7.