PMID- 10341242
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 11
DP  - 1999 Jun 1
TI  - Floor plate and netrin-1 are involved in the migration and survival of inferior
      olivary neurons.
PG  - 4407-20
AB  - During their circumferential migration, the nuclei of inferior olivary neurons
      translocate within their axons until they reach the floor plate where they stop, 
      although their axons have already crossed the midline to project to the
      contralateral cerebellum. Signals released by the floor plate, including
      netrin-1, have been implicated in promoting axonal growth and chemoattraction
      during axonal pathfinding in different midline crossing systems. In the present
      study, we report experiments that strongly suggest that the floor plate could
      also be involved in the migration of inferior olivary neurons. First, we show
      that the pattern of expression of netrin receptors DCC (for deleted in colorectal
      cancer), neogenin (a DCC-related protein), and members of the Unc5 family in
      wild-type mice is consistent with a possible role of netrins in directing the
      migration of precerebellar neurons from the rhombic lips. Second, we have studied
      mice deficient in netrin-1 production. In these mice, the number of inferior
      olivary neurons is remarkably decreased. Some of them are located ectopically
      along the migration stream, whereas the others are located medioventrally and
      form an atrophic inferior olivary complex: most subnuclei are missing. However,
      axons of the remaining olivary cell bodies located in the vicinity of the floor
      plate still succeed in entering their target, the cerebellum, but they establish 
      an ipsilateral projection instead of the normal contralateral projection. In
      vitro experiments involving ablations of the midline show a fusion of the two
      olivary masses normally located on either side of the ventral midline, suggesting
      that the floor plate may function as a specific stop signal for inferior olivary 
      neurons. These results establish a requirement for netrin-1 in the migration of
      inferior olivary neurons and suggest that it may function as a specific guidance 
      cue for the initial steps of the migration from the rhombic lips and then later
      in the development of the normal crossed projection of the inferior olivary
      neurons. They also establish a requirement for netrin-1, either directly or
      indirectly, for the survival of inferior olivary neurons.
FAU - Bloch-Gallego, E
AU  - Bloch-Gallego E
AD  - Institut National de la Sante et de la Recherche Medicale U106, Hopital de la
      Salpetriere, 75013 Paris, France.
FAU - Ezan, F
AU  - Ezan F
FAU - Tessier-Lavigne, M
AU  - Tessier-Lavigne M
FAU - Sotelo, C
AU  - Sotelo C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Ntn1 protein, mouse)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 158651-98-0 (Netrin-1)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Movement/physiology
MH  - Cell Survival/physiology
MH  - Cerebellum/physiology
MH  - Chick Embryo
MH  - Mice
MH  - Mutation
MH  - Nerve Growth Factors/*physiology
MH  - Nervous System/*embryology
MH  - Netrin-1
MH  - Neural Pathways/physiology
MH  - Neurons/cytology/*physiology
MH  - Olivary Nucleus/cytology/*physiology
MH  - Organ Culture Techniques
MH  - Purkinje Cells/physiology
MH  - Tumor Suppressor Proteins
PMC - PMC6782586
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Jun 1;19(11):4407-20.