PMID- 10341223
OWN - NLM
STAT- MEDLINE
DCOM- 19990616
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 11
DP  - 1999 Jun 1
TI  - Characterization of MALS/Velis-1, -2, and -3: a family of mammalian LIN-7
      homologs enriched at brain synapses in association with the postsynaptic
      density-95/NMDA receptor postsynaptic complex.
PG  - 4189-99
AB  - Protein assembly at the postsynaptic density (PSD) of neuronal synapses is
      mediated in part by protein interactions with PSD-95/discs large/zona occludens-1
      (PDZ) motifs. Here, we identify MALS-1, -2, -3, a family of small synaptic
      proteins containing little more than a single PDZ domain. MALS-1, -2, and -3 are 
      mammalian homologs LIN-7, a Caenorhabditis elegans protein essential for vulval
      development. In contrast to functions for LIN-7 in epithelial cells, MALS-1 and
      -2 are selectively expressed in specific neuronal populations in brain and are
      enriched in PSD fractions. In cultured hippocampal neurons, MALS proteins are
      clustered together with PSD-95 and NMDA type glutamate receptors, consistent with
      a postsynaptic localization for MALS proteins. Immunoprecipitation and affinity
      chromatography studies readily identify association of MALS with PSD-95 and an
      NMDA receptor subunit. The PDZ domain of MALS selectively binds to peptides
      terminating in E-T/S-R/X-V/I/L, which corresponds to the C terminus of NMDA type 
      2 receptors and numerous other ion channels at the PSD. This work suggests a role
      for MALS proteins in regulating recruitment of neurotransmitter receptors to the 
      PSD.
FAU - Jo, K
AU  - Jo K
AD  - Department of Physiology, School of Medicine, University of California at San
      Francisco, San Francisco, California 94143-0444, USA.
FAU - Derin, R
AU  - Derin R
FAU - Li, M
AU  - Li M
FAU - Bredt, D S
AU  - Bredt DS
LA  - eng
SI  - GENBANK/AF173081
SI  - GENBANK/AF173082
SI  - GENBANK/AF173083
GR  - R01 NS033324/NS/NINDS NIH HHS/United States
GR  - R01 GM36017/GM/NIGMS NIH HHS/United States
GR  - NS33324/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LIN-7 protein, C elegans)
RN  - 0 (LIN-7 protein, mammalian)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (postsynaptic density proteins)
RN  - EC 3.2.1.- (Amylases)
RN  - EC 3.2.1.- (MalS protein, E coli)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amylases/*analysis/genetics
MH  - Animals
MH  - Brain Chemistry/*physiology
MH  - COS Cells
MH  - *Caenorhabditis elegans Proteins
MH  - Disks Large Homolog 4 Protein
MH  - *Escherichia coli Proteins
MH  - Helminth Proteins/*analysis
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins/*analysis
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Nerve Tissue Proteins/*analysis/chemistry/genetics
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/chemistry
MH  - Sequence Homology, Amino Acid
MH  - Synapses/*chemistry
PMC - PMC6782594
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Jun 1;19(11):4189-99.