PMID- 10340301
OWN - NLM
STAT- MEDLINE
DCOM- 19990721
LR  - 20191210
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 38
IP  - 5
DP  - 1999 May
TI  - The protein kinase C alpha binding protein PICK1 interacts with short but not
      long form alternative splice variants of AMPA receptor subunits.
PG  - 635-44
AB  - Here we report an interaction between AMPA receptor subunits and a single PDZ
      domain-containing protein called PICK1 which is known to bind protein kinase C
      alpha (PKC alpha). The interaction occurs within the last ten amino acid residues
      containing a novel PDZ binding motif (E S V/I K I) of the short C-terminal
      alternative splice variants of AMPA receptor subunits. No interaction occurs with
      the corresponding long splice variants which do not contain the E S V/I K I
      motif. The PDZ domain of PICK1 is required for the interaction and the mutation
      of a single amino acid in this region (Lys-27 to Glu) prevents interaction
      between PICK1 and GluR2 in the yeast two-hybrid assay. A similar mutation has
      been reported to prevent the binding of PICK1 to PKC alpha indicating that the
      same domain of PICK1 binds both PKC alpha and GluRs. Flag-tagged PICK1 is
      retained by a glutathione S-transferase (GST) fusion of the C-terminal of GluR2
      (GST-ct-GluR2; short splice variant) but not by GST-ct-GluR1 (long splice
      variant). Recombinant full length GluR2 is coimmunoprecipitated with flag-PICK1
      using an anti-flag antibody and flag-PICK1 is coimmunoprecipitated with an
      N-terminal directed anti-GluR2 antibody. Transient expression of both proteins in
      COS cells reveals colocalization and an altered pattern of distribution for each 
      protein from when they are expressed individually. This novel interaction
      provides a possible regulatory mechanism to specifically modulate distinct splice
      variants and may be involved in targeting the phosphorylation of short form GluRs
      by PKC alpha.
FAU - Dev, K K
AU  - Dev KK
AD  - Department of Biological Sciences, Kyoto University, Faculty of Medicine, Japan.
FAU - Nishimune, A
AU  - Nishimune A
FAU - Henley, J M
AU  - Henley JM
FAU - Nakanishi, S
AU  - Nakanishi S
LA  - eng
GR  - G9629038/Medical Research Council/United Kingdom
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Prkcabp protein, rat)
RN  - 0 (Receptors, AMPA)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - P6W5IXV8V9 (glutamate receptor ionotropic, AMPA 2)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Carrier Proteins/*metabolism
MH  - Cytoskeletal Proteins
MH  - Isoenzymes/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - Protein Kinase C-alpha
MH  - Rats
MH  - Receptors, AMPA/*genetics/*metabolism
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - S0028390898002305 [pii]
AID - 10.1016/s0028-3908(98)00230-5 [doi]
PST - ppublish
SO  - Neuropharmacology. 1999 May;38(5):635-44. doi: 10.1016/s0028-3908(98)00230-5.