PMID- 10339604
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20190816
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 11
DP  - 1999 May 25
TI  - MSF (MLL septin-like fusion), a fusion partner gene of MLL, in a therapy-related 
      acute myeloid leukemia with a t(11;17)(q23;q25).
PG  - 6428-33
AB  - MLL (ALL1, Htrx, HRX), which is located on chromosome band 11q23, frequently is
      rearranged in patients with therapy-related acute myeloid leukemia who previously
      were treated with DNA topoisomerase II inhibitors. In this study, we have
      identified a fusion partner of MLL in a 10-year-old female who developed
      therapy-related acute myeloid leukemia 17 months after treatment for Hodgkin's
      disease. Leukemia cells of this patient had a t(11;17)(q23;q25), which involved
      MLL as demonstrated by Southern blot analysis. The partner gene was cloned from
      cDNA of the leukemia cells by use of a combination of adapter reverse
      transcriptase-PCR, rapid amplification of 5' cDNA ends, and BLAST database
      analysis to identify expressed sequence tags. The full-length cDNA of 2.8 kb was 
      found to be an additional member of the septin family, therefore it was named MSF
      (MLL septin-like fusion). Members of the septin family conserve the GTP binding
      domain, localize in the cytoplasm, and interact with cytoskeletal filaments. A
      major 4-kb transcript of MSF was expressed ubiquitously; a 1.7-kb transcript was 
      found in most tissues. An additional 3-kb transcript was found only in
      hematopoietic tissues. By amplification with MLL exon 5 forward primer and
      reverse primers in MSF, the appropriately sized products were obtained. MSF is
      highly homologous to hCDCrel-1, which is a partner gene of MLL in leukemias with 
      a t(11;22)(q23;q11.2). Further analysis of MSF may help to delineate the function
      of MLL partner genes in leukemia, particularly in therapy-related leukemia.
FAU - Osaka, M
AU  - Osaka M
AD  - Section of Hematology/Oncology, Department of Medicine, University of Chicago,
      Chicago, IL 60637, USA.
FAU - Rowley, J D
AU  - Rowley JD
FAU - Zeleznik-Le, N J
AU  - Zeleznik-Le NJ
LA  - eng
SI  - GENBANK/AF123052
GR  - P01 CA040046/CA/NCI NIH HHS/United States
GR  - CA42557/CA/NCI NIH HHS/United States
GR  - P01 CA40046/CA/NCI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - 35S93Y190K (Procarbazine)
RN  - 5J49Q6B70F (Vincristine)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.1.- (SEPTIN9 protein, human)
RN  - EC 3.6.1.- (Septins)
RN  - VB0R961HZT (Prednisone)
RN  - COPP protocol
SB  - IM
MH  - Acute Disease
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antineoplastic Combined Chemotherapy Protocols/*adverse effects
MH  - Base Sequence
MH  - Child
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 17
MH  - Cyclophosphamide/administration & dosage
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila/genetics
MH  - Female
MH  - *GTP Phosphohydrolases
MH  - GTP-Binding Proteins/chemistry/*genetics
MH  - Histone-Lysine N-Methyltransferase
MH  - Hodgkin Disease/*drug therapy
MH  - Humans
MH  - Leukemia, Myeloid/*chemically induced/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Organ Specificity
MH  - Prednisone/administration & dosage
MH  - Procarbazine/administration & dosage
MH  - *Proto-Oncogenes
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Septins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - *Transcription Factors
MH  - *Translocation, Genetic
MH  - Vincristine/administration & dosage
MH  - Zinc Fingers
PMC - PMC26898
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1073/pnas.96.11.6428 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 May 25;96(11):6428-33. doi:
      10.1073/pnas.96.11.6428.