PMID- 10339590
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 11
DP  - 1999 May 25
TI  - Receptors for oxidized low-density lipoprotein on elicited mouse peritoneal
      macrophages can recognize both the modified lipid moieties and the modified
      protein moieties: implications with respect to macrophage recognition of
      apoptotic cells.
PG  - 6347-52
AB  - It has been shown previously that the binding of oxidized low-density lipoprotein
      (OxLDL) to resident mouse peritoneal macrophages can be inhibited (up to 70%) by 
      the apoprotein B (apoB) isolated from OxLDL, suggesting that macrophage
      recognition of OxLDL is primarily dependent on its modified protein moiety.
      However, recent experiments have demonstrated that the lipids isolated from OxLDL
      and reconstituted into a microemulsion can also strongly inhibit uptake of OxLDL 
      (up to 80%). The present studies show that lipid microemulsions prepared from
      OxLDL bind to thioglycollate-elicited macrophages at 4 degrees C in a saturable
      fashion and inhibit the binding of intact OxLDL and also of the apoB from OxLDL. 
      Reciprocally, the binding of the OxLDL-lipid microemulsions was strongly
      inhibited by intact OxLDL. A conjugate of synthetic 1-palmitoyl 2(5-oxovaleroyl) 
      phosphatidylcholine (an oxidation product of 1-palmitoyl 2-arachidonoyl
      phosphatidylcholine) with serum albumin, shown previously to inhibit macrophage
      binding of intact OxLDL, also inhibited the binding of both the apoprotein and
      the lipid microemulsions prepared from OxLDL. Finally, a monoclonal antibody
      against oxidized phospholipids, one that inhibits binding of intact OxLDL to
      macrophages, also inhibited the binding of both the resolubilized apoB and the
      lipid microemulsions prepared from OxLDL. These studies support the conclusions
      that: (i) at least some of the macrophage receptors for oxidized LDL can
      recognize both the lipid and the protein moieties; and (ii) oxidized
      phospholipids, in the lipid phase of the lipoprotein and/or covalently linked to 
      the apoB of OxLDL, likely play a role in that recognition.
FAU - Bird, D A
AU  - Bird DA
AD  - Department of Medicine 0682, University of California, San Diego, 9500 Gilman
      Drive, La Jolla, CA 92093-0682, USA.
FAU - Gillotte, K L
AU  - Gillotte KL
FAU - Horkko, S
AU  - Horkko S
FAU - Friedman, P
AU  - Friedman P
FAU - Dennis, E A
AU  - Dennis EA
FAU - Witztum, J L
AU  - Witztum JL
FAU - Steinberg, D
AU  - Steinberg D
LA  - eng
GR  - R01 HL057505/HL/NHLBI NIH HHS/United States
GR  - T32 DK007044/DK/NIDDK NIH HHS/United States
GR  - HL56989/HL/NHLBI NIH HHS/United States
GR  - P50 HL056989/HL/NHLBI NIH HHS/United States
GR  - T32 HL007276/HL/NHLBI NIH HHS/United States
GR  - DK07044/DK/NIDDK NIH HHS/United States
GR  - HL57505/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (1-palmitoyl-2-(5-oxovaleroyl)phosphatidylcholine)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Emulsions)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Liposomes)
RN  - 0 (OLR1 protein, human)
RN  - 0 (Olr1 protein, mouse)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Receptors, LDL)
RN  - 0 (Receptors, Oxidized LDL)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 0 (oxidized low density lipoprotein)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Apolipoproteins B/metabolism
MH  - Apoptosis
MH  - Cells, Cultured
MH  - Emulsions
MH  - Female
MH  - Humans
MH  - Kinetics
MH  - Lipoproteins, LDL/blood/isolation & purification/*metabolism
MH  - Liposomes
MH  - Macrophages, Peritoneal/cytology/*physiology
MH  - Mice
MH  - Phosphatidylcholines/chemical synthesis/*pharmacokinetics
MH  - Receptors, LDL/*physiology
MH  - Receptors, Oxidized LDL
MH  - Scavenger Receptors, Class E
MH  - Serum Albumin, Bovine/pharmacokinetics
MH  - Substrate Specificity
PMC - PMC26884
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1073/pnas.96.11.6347 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 May 25;96(11):6347-52. doi:
      10.1073/pnas.96.11.6347.