PMID- 10339552
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 11
DP  - 1999 May 25
TI  - The SRm160/300 splicing coactivator is required for exon-enhancer function.
PG  - 6125-30
AB  - Exonic splicing enhancer (ESE) sequences are important for the recognition of
      splice sites in pre-mRNA. These sequences are bound by specific serine-arginine
      (SR) repeat proteins that promote the assembly of splicing complexes at adjacent 
      splice sites. We have recently identified a splicing "coactivator," SRm160/300,
      which contains SRm160 (the SR nuclear matrix protein of 160 kDa) and a 300-kDa
      nuclear matrix antigen. In the present study, we show that SRm160/300 is required
      for a purine-rich ESE to promote the splicing of a pre-mRNA derived from the
      Drosophila doublesex gene. The association of SRm160/300 and U2 small nuclear
      ribonucleoprotein particle (snRNP) with this pre-mRNA requires both U1 snRNP and 
      factors bound to the ESE. Independently of pre-mRNA, SRm160/300 specifically
      interacts with U2 snRNP and with a human homolog of the Drosophila alternative
      splicing regulator Transformer 2, which binds to purine-rich ESEs. The results
      suggest a model for ESE function in which the SRm160/300 splicing coactivator
      promotes critical interactions between ESE-bound "activators" and the snRNP
      machinery of the spliceosome.
FAU - Eldridge, A G
AU  - Eldridge AG
AD  - Banting and Best Department of Medical Research, University of Toronto, Toronto, 
      ON M5G 1L6, Canada.
FAU - Li, Y
AU  - Li Y
FAU - Sharp, P A
AU  - Sharp PA
FAU - Blencowe, B J
AU  - Blencowe BJ
LA  - eng
GR  - P30 CA014051/CA/NCI NIH HHS/United States
GR  - P30-CA14051/CA/NCI NIH HHS/United States
GR  - R37-GM34277/GM/NIGMS NIH HHS/United States
GR  - R37 GM034277/GM/NIGMS NIH HHS/United States
GR  - RI1-AI32486/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens)
RN  - 0 (Antigens, Nuclear)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DSX protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoprotein, U1 Small Nuclear)
RN  - 0 (Ribonucleoprotein, U2 Small Nuclear)
RN  - 0 (SRRM1 protein, human)
SB  - IM
MH  - Animals
MH  - Antigens/metabolism
MH  - *Antigens, Nuclear
MH  - Cell Nucleus/*metabolism
MH  - DNA-Binding Proteins/genetics
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/metabolism
MH  - *Enhancer Elements, Genetic
MH  - *Exons
MH  - HeLa Cells
MH  - Humans
MH  - Insect Proteins/genetics
MH  - *Nuclear Matrix-Associated Proteins
MH  - Nuclear Proteins/*metabolism
MH  - RNA Precursors/chemistry/genetics/metabolism
MH  - *RNA Splicing
MH  - RNA-Binding Proteins/*metabolism
MH  - Ribonucleoprotein, U1 Small Nuclear/genetics/metabolism
MH  - Ribonucleoprotein, U2 Small Nuclear/genetics/metabolism
PMC - PMC26846
EDAT- 1999/05/26 00:00
MHDA- 1999/05/26 00:01
CRDT- 1999/05/26 00:00
PHST- 1999/05/26 00:00 [pubmed]
PHST- 1999/05/26 00:01 [medline]
PHST- 1999/05/26 00:00 [entrez]
AID - 10.1073/pnas.96.11.6125 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 May 25;96(11):6125-30. doi:
      10.1073/pnas.96.11.6125.