PMID- 10338214
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 4
DP  - 1999 May 14
TI  - Mammalian telomeres end in a large duplex loop.
PG  - 503-14
AB  - Mammalian telomeres contain a duplex array of telomeric repeats bound to the
      telomeric repeat-binding factors TRF1 and TRF2. Inhibition of TRF2 results in
      immediate deprotection of chromosome ends, manifested by loss of the telomeric 3'
      overhang, activation of p53, and end-to-end chromosome fusions. Electron
      microscopy reported here demonstrated that TRF2 can remodel linear telomeric DNA 
      into large duplex loops (t loops) in vitro. Electron microscopy analysis of
      psoralen cross-linked telomeric DNA purified from human and mouse cells revealed 
      abundant large t loops with a size distribution consistent with their telomeric
      origin. Binding of TRF1 and single strand binding protein suggested that t loops 
      are formed by invasion of the 3' telomeric overhang into the duplex telomeric
      repeat array. T loops may provide a general mechanism for the protection and
      replication of telomeres.
FAU - Griffith, J D
AU  - Griffith JD
AD  - Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel
      Hill and Curriculum in Genetics and Molecular Biology, 27599-7295, USA.
      jdg@med.unc.edu
FAU - Comeau, L
AU  - Comeau L
FAU - Rosenfield, S
AU  - Rosenfield S
FAU - Stansel, R M
AU  - Stansel RM
FAU - Bianchi, A
AU  - Bianchi A
FAU - Moss, H
AU  - Moss H
FAU - de Lange, T
AU  - de Lange T
LA  - eng
GR  - CA19043/CA/NCI NIH HHS/United States
GR  - GM31819/GM/NIGMS NIH HHS/United States
GR  - GM49046/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Telomeric Repeat Binding Protein 1)
RN  - 0 (Telomeric Repeat Binding Protein 2)
RN  - 9007-49-2 (DNA)
SB  - IM
CIN - Cell. 1999 May 14;97(4):419-22. PMID: 10338204
MH  - Animals
MH  - Cross-Linking Reagents
MH  - DNA/metabolism/*ultrastructure
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mammals
MH  - Mice
MH  - *Models, Molecular
MH  - Nucleic Acid Heteroduplexes
MH  - Recombinant Fusion Proteins/metabolism
MH  - Telomere/metabolism/*ultrastructure
MH  - Telomeric Repeat Binding Protein 1
MH  - Telomeric Repeat Binding Protein 2
EDAT- 1999/05/25 00:00
MHDA- 1999/05/25 00:01
CRDT- 1999/05/25 00:00
PHST- 1999/05/25 00:00 [pubmed]
PHST- 1999/05/25 00:01 [medline]
PHST- 1999/05/25 00:00 [entrez]
AID - S0092-8674(00)80760-6 [pii]
AID - 10.1016/s0092-8674(00)80760-6 [doi]
PST - ppublish
SO  - Cell. 1999 May 14;97(4):503-14. doi: 10.1016/s0092-8674(00)80760-6.