PMID- 10337625
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 5
DP  - 1999 May
TI  - Cloning and characterization of murine Aqp5: evidence for a conserved aquaporin
      gene cluster.
PG  - 498-505
AB  - Aquaporin 5 (Aqp5), a member of the aquaporin family of membrane water channels, 
      is thought to modulate the osmolality of fluids in the eye, lung, and salivary
      gland. Here, we report the cloning and genomic characterization of murine Aqp5
      and its expression in relevant mouse tissues. This gene, comprised of four exons 
      encoding 265 amino acids (121, 55, 28, and 61 amino acids respectively), is
      transcribed into an approximate 1.8-kb mRNA detected in lung, parotid,
      submandibular, sublingual, and lacrimal tissues. Aqp5 encodes a protein that is
      98% identical to rat Aqp5. An Aqp5 antibody detects an approximately 27-kDa
      protein band in mouse lung, and an additional 29 kDa band in salivary gland.
      Cloning and physical mapping genomic fragments contiguous with Aqp5 revealed two 
      other members of the aquaporin family: Aqp2 and Aqp6, arrayed head to tail in the
      order Aqp2-Aqp5-Aqp6, and provides evidence of a gene cluster conserved in order 
      and orientation in both mice and humans.
FAU - Krane, C M
AU  - Krane CM
AD  - Department of Molecular Genetics, Biochemistry, and Microbiology, University of
      Cincinnati College of Medicine, Ohio 45267-0524, USA.
FAU - Towne, J E
AU  - Towne JE
FAU - Menon, A G
AU  - Menon AG
LA  - eng
SI  - GENBANK/AF087654
SI  - GENBANK/AF087655
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (AQP5 protein, human)
RN  - 0 (Aqp5 protein, mouse)
RN  - 0 (Aqp5 protein, rat)
RN  - 0 (Aquaporin 5)
RN  - 0 (Aquaporins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aquaporin 5
MH  - Aquaporins/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary
MH  - Exons
MH  - Humans
MH  - Introns
MH  - *Membrane Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Rats
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/25 00:00
MHDA- 1999/05/25 00:01
CRDT- 1999/05/25 00:00
PHST- 1999/05/25 00:00 [pubmed]
PHST- 1999/05/25 00:01 [medline]
PHST- 1999/05/25 00:00 [entrez]
AID - 10.1007/s003359901030 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 May;10(5):498-505. doi: 10.1007/s003359901030.