PMID- 10336991
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 6
DP  - 1999 Jun
TI  - Structure of the human gene for lysosomal di-N-acetylchitobiase.
PG  - 589-93
AB  - Chitobiase is a lysosomal glycosidase that acts during the ordered degradation of
      asparagine-linked glycoproteins to cleave the core chitobiose unit at its
      reducing end. Human chitobiase is expressed in significant amounts, while bovine 
      chitobiase is produced at extremely low levels. To begin to understand this
      species-dependent expression, we determined the gene structure of human
      chitobiase. The human chitobiase gene ( CTB S) is approximately 20 kb comprising 
      seven exons varying from 0.1 to 2.3 kb and six introns of 0.3 to 8 kb. The
      previously characterized partial bovine chitobiase gene structure is similarly
      organized including exon and intron sizes and locations, but the human and bovine
      5'-flanking regions differ significantly. 5'-RACE analysis of human chitobiase
      cDNA revealed only one transcriptional start site 45 bp upstream of the ATG
      translation initiation site. Computer analysis of the human chitobiase gene
      5'-flanking region shows characteristics of a typical housekeeping gene. The
      putative promoter region contains a distal TATA box, and there are several Sp-1
      and AP-2 cis elements. In contrast, bovine chitobiase gene 5'-flanking region
      shows totally different structures and may contain several silencers. A partial
      art-2 segment which is an artiodactyl Alu -like repetitive sequence, is also
      present. These evolutionary differences in the 5'-flanking region of the
      chitobiase genes from human and bovine could account for the widely varied
      expression levels of the hydrolase within these two species.
FAU - Liu, B
AU  - Liu B
AD  - Department of Biochemistry and Molecular Biology, College of Medicine, University
      of South Alabama, Mobile, AL 36688, USA.
FAU - Ahmad, W
AU  - Ahmad W
FAU - Aronson, N N Jr
AU  - Aronson NN Jr
LA  - eng
PT  - Journal Article
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (DNA, Recombinant)
RN  - 0 (Disaccharides)
RN  - 2706-64-1 (N,N-diacetylchitobiose)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - DNA, Recombinant
MH  - Disaccharides/*genetics
MH  - Humans
MH  - Lysosomes/*enzymology
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/25 06:00
MHDA- 2001/03/28 10:01
CRDT- 1999/05/25 06:00
PHST- 1999/05/25 06:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/05/25 06:00 [entrez]
AID - cwc052 [pii]
AID - 10.1093/glycob/9.6.589 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Jun;9(6):589-93. doi: 10.1093/glycob/9.6.589.