PMID- 10334869
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20181201
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 366
IP  - 1
DP  - 1999 Jun 1
TI  - Molecular and functional characterization of the intestinal Na+-dependent
      multivitamin transporter.
PG  - 95-106
AB  - We have cloned a Na+-dependent multivitamin transporter from rabbit intestine
      (riSMVT). The cDNA codes for a protein of 636 amino acids with 12 putative
      transmembrane domains. When expressed in mammalian cells, the cDNA induces
      Na+-dependent uptake of the vitamins pantothenate and biotin. Lipoate is also a
      substrate for the cDNA-induced uptake process. The affinity constant for the
      cDNA-specific transport of pantothenate and biotin is approximately 2 and
      approximately 8 microM, respectively. The Na+:vitamin stoichiometry is greater
      than 1, indicating that the transport process is electrogenic. The SMVT-specific 
      transcripts of 3.2 kbp are equally distributed throughout the small intestine. We
      have also cloned SMVT from the human intestinal cell line Caco-2. The Caco-2 SMVT
      cDNA codes for a protein of 635 amino acids which is homologous to riSMVT and is 
      identical to the SMVT expressed in the human choriocarcinoma cell line JAR.
      Caco-2 SMVT also catalyzes Na+-dependent uptake of pantothenate, biotin, and
      lipoate. In oocytes expressing Caco-2 SMVT, all three vitamins evoke inward
      currents, confirming the electrogenicity of the transport process.
CI  - Copyright 1999 Academic Press.
FAU - Prasad, P D
AU  - Prasad PD
AD  - Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta,
      Georgia, 30912, USA. pprasad@mail.mcg.edu
FAU - Wang, H
AU  - Wang H
FAU - Huang, W
AU  - Huang W
FAU - Fei, Y J
AU  - Fei YJ
FAU - Leibach, F H
AU  - Leibach FH
FAU - Devoe, L D
AU  - Devoe LD
FAU - Ganapathy, V
AU  - Ganapathy V
LA  - eng
SI  - GENBANK/AF080067
SI  - GENBANK/AF081571
GR  - HD 33347/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Symporters)
RN  - 0 (biotin transporter)
RN  - 19F5HK2737 (Pantothenic Acid)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biotin/*metabolism
MH  - Caco-2 Cells
MH  - Carrier Proteins/biosynthesis/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Intestinal Mucosa/*metabolism
MH  - Membrane Glycoproteins/biosynthesis/*genetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Pantothenic Acid/*metabolism
MH  - Protein Conformation
MH  - Rabbits
MH  - Recombinant Proteins/biosynthesis
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - *Symporters
MH  - Xenopus laevis
EDAT- 1999/05/21 00:00
MHDA- 1999/05/21 00:01
CRDT- 1999/05/21 00:00
PHST- 1999/05/21 00:00 [pubmed]
PHST- 1999/05/21 00:01 [medline]
PHST- 1999/05/21 00:00 [entrez]
AID - S0003-9861(99)91213-4 [pii]
AID - 10.1006/abbi.1999.1213 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Jun 1;366(1):95-106. doi: 10.1006/abbi.1999.1213.