PMID- 10332029
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20201209
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 8
IP  - 6
DP  - 1999 Jun
TI  - PQBP-1, a novel polyglutamine tract-binding protein, inhibits transcription
      activation by Brn-2 and affects cell survival.
PG  - 977-87
AB  - A novel gene, designated PQBP-1, which encodes a 265 residue protein that binds
      to the polyglutamine tract of the brain-specific transcription factor Brn-2, was 
      identified. PQBP-1, which also interacts with the polyglutamine tract of triplet 
      repeat disease gene products, binds with a higher affinity to an expanded
      polyglutamine tract. PQBP-1 has several functional domains, including hepta- and 
      di-amino acid repeat sequences rich in polar residues essential for its
      interaction with the polyglutamine tract, a WWP/WW domain which binds to
      proline-rich motifs in other proteins, a putative nuclear localization signal
      sequence and a C2domain implicated in Ca2+-dependent phospholipid signaling.
      PQBP-1 is located in the nucleus and inhibits transcriptional activation by
      Brn-2. Overexpression of PQBP-1 in P19 embryonic carcinoma cells suppresses their
      growth rate and enhances their susceptibility to various stresses including serum
      deprivation, retinoic acid treatment and UV irradiation. Northern blot and in
      situ hybridization analyses revealed that PQBP-1 is a ubiquitous protein and is
      expressed primarily in neurons throughout the brain, with abundant levels in
      hippocampus, cerebellar cortex and olfactory bulb. These results suggest that
      PQBP-1 mediates important cellular functions under physiological and pathological
      conditions via its interaction with polyglutamine tracts.
FAU - Waragai, M
AU  - Waragai M
AD  - Group of Molecular Neurobiology, Department of Neurology, Graduate School of
      Medicine, University of Tokyo, Japan.
FAU - Lammers, C H
AU  - Lammers CH
FAU - Takeuchi, S
AU  - Takeuchi S
FAU - Imafuku, I
AU  - Imafuku I
FAU - Udagawa, Y
AU  - Udagawa Y
FAU - Kanazawa, I
AU  - Kanazawa I
FAU - Kawabata, M
AU  - Kawabata M
FAU - Mouradian, M M
AU  - Mouradian MM
FAU - Okazawa, H
AU  - Okazawa H
LA  - eng
SI  - GENBANK/AJ242829
SI  - GENBANK/AJ250406
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (POU Domain Factors)
RN  - 0 (PQBP1 protein, human)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (transcription factor Brn-2)
RN  - 26700-71-0 (polyglutamine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Brain/metabolism
MH  - Carrier Proteins/genetics/metabolism/*physiology
MH  - Cell Nucleus/chemistry
MH  - Cell Survival/physiology
MH  - DNA/genetics/metabolism
MH  - DNA, Complementary/chemistry/genetics
MH  - DNA-Binding Proteins
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Homeodomain Proteins
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism/*physiology
MH  - POU Domain Factors
MH  - Peptides/*metabolism
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Analysis, DNA
MH  - Tissue Distribution
MH  - Transcription Factors/*physiology
MH  - Transcriptional Activation
MH  - Trinucleotide Repeats/genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - ddc126 [pii]
AID - 10.1093/hmg/8.6.977 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1999 Jun;8(6):977-87. doi: 10.1093/hmg/8.6.977.