PMID- 10331393
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20220316
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 399
IP  - 6731
DP  - 1999 May 6
TI  - Regulation of alternative splicing by RNA editing.
PG  - 75-80
AB  - The enzyme ADAR2 is a double-stranded RNA-specific adenosine deaminase which is
      involved in the editing of mammalian messenger RNAs by the site-specific
      conversion of adenosine to inosine. Here we identify several rat ADAR2 mRNAs
      produced as a result of two distinct alternative splicing events. One such
      splicing event uses a proximal 3' acceptor site, adding 47 nucleotides to the
      ADAR2 coding region, changing the predicted reading frame of the mature ADAR2
      transcript. Nucleotide-sequence analysis of ADAR2 genomic DNA revealed the
      presence of adenosine-adenosine (AA) and adenosine-guanosine (AG) dinucleotides
      at these proximal and distal alternative 3' acceptor sites, respectively. Use of 
      the proximal 3' acceptor depends upon the ability of ADAR2 to edit its own
      pre-mRNA, converting the intronic AA to an adenosine-inosine (AI) dinucleotide
      which effectively mimics the highly conserved AG sequence normally found at 3'
      splice junctions. Our observations indicate that RNA editing can serve as a
      mechanism for regulating alternative splicing and they suggest a novel strategy
      by which ADAR2 can modulate its own expression.
FAU - Rueter, S M
AU  - Rueter SM
AD  - Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, 
      Tennessee 37232-6600, USA.
FAU - Dawson, T R
AU  - Dawson TR
FAU - Emeson, R B
AU  - Emeson RB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 12133JR80S (Guanosine)
RN  - 9007-49-2 (DNA)
RN  - EC 3.5.4.4 (ADARB1 protein, human)
RN  - EC 3.5.4.4 (Adenosine Deaminase)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/metabolism
MH  - Adenosine Deaminase/*genetics
MH  - Alternative Splicing/*physiology
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA
MH  - Gene Expression Regulation, Enzymologic
MH  - Guanosine/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - RNA Editing/*physiology
MH  - RNA, Messenger/genetics
MH  - RNA-Binding Proteins
MH  - Rats
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/05/20 06:00
MHDA- 2001/03/23 10:01
CRDT- 1999/05/20 06:00
PHST- 1999/05/20 06:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/20 06:00 [entrez]
AID - 10.1038/19992 [doi]
PST - ppublish
SO  - Nature. 1999 May 6;399(6731):75-80. doi: 10.1038/19992.