PMID- 10331392
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 399
IP  - 6731
DP  - 1999 May 6
TI  - A family of mammalian Na+-dependent L-ascorbic acid transporters.
PG  - 70-5
AB  - Vitamin C (L-ascorbic acid) is essential for many enzymatic reactions, in which
      it serves to maintain prosthetic metal ions in their reduced forms (for example, 
      Fe2+, Cu+), and for scavenging free radicals in order to protect tissues from
      oxidative damage. The facilitative sugar transporters of the GLUT type can
      transport the oxidized form of the vitamin, dehydroascorbic acid, but these
      transporters are unlikely to allow significant physiological amounts of vitamin C
      to be taken up in the presence of normal glucose concentrations, because the
      vitamin is present in plasma essentially only in its reduced form. Here we
      describe the isolation of two L-ascorbic acid transporters, SVCT1 and SVCT2, from
      rat complementary DNA libraries, as the first step in investigating the
      importance of L-ascorbic acid transport in regulating the supply and metabolism
      of vitamin C. We find that SVCT1 and SVCT2 each mediate concentrative,
      high-affinity L-ascorbic acid transport that is stereospecific and is driven by
      the Na+ electrochemical gradient. Despite their close sequence homology and
      similar functions, the two isoforms of the transporter are discretely
      distributed: SVCT1 is mainly confined to epithelial systems (intestine, kidney,
      liver), whereas SVCT2 serves a host of metabolically active cells and specialized
      tissues in the brain, eye and other organs.
FAU - Tsukaguchi, H
AU  - Tsukaguchi H
AD  - Membrane Biology Program, Department of Medicine, Brigham & Women's Hospital and 
      Harvard Medical School, Boston, Massachusetts 02115, USA.
FAU - Tokui, T
AU  - Tokui T
FAU - Mackenzie, B
AU  - Mackenzie B
FAU - Berger, U V
AU  - Berger UV
FAU - Chen, X Z
AU  - Chen XZ
FAU - Wang, Y
AU  - Wang Y
FAU - Brubaker, R F
AU  - Brubaker RF
FAU - Hediger, M A
AU  - Hediger MA
LA  - eng
SI  - GENBANK/AF080452
SI  - GENBANK/AF080453
SI  - GENBANK/AF118561
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (DNA, Complementary)
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Proteins)
RN  - 0 (Slc23a1 protein, rat)
RN  - 0 (Slc23a2 protein, rat)
RN  - 0 (Sodium-Coupled Vitamin C Transporters)
RN  - 0 (Symporters)
RN  - 9NEZ333N27 (Sodium)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Ascorbic Acid/*metabolism
MH  - Biological Transport
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Molecular Sequence Data
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - Proteins/genetics/*isolation & purification/metabolism
MH  - Rabbits
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Sodium/*metabolism
MH  - Sodium-Coupled Vitamin C Transporters
MH  - *Symporters
MH  - Tissue Distribution
MH  - Xenopus
EDAT- 1999/05/20 06:00
MHDA- 2001/03/23 10:01
CRDT- 1999/05/20 06:00
PHST- 1999/05/20 06:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/20 06:00 [entrez]
AID - 10.1038/19986 [doi]
PST - ppublish
SO  - Nature. 1999 May 6;399(6731):70-5. doi: 10.1038/19986.