PMID- 10330415
OWN - NLM
STAT- MEDLINE
DCOM- 19990721
LR  - 20191210
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 4
DP  - 1999 May 17
TI  - Sulfotransferases of two specificities function in the reconstitution of high
      endothelial cell ligands for L-selectin.
PG  - 899-910
AB  - L-selectin, a lectin-like receptor, mediates rolling of lymphocytes on high
      endothelial venules (HEVs) in secondary lymphoid organs by interacting with HEV
      ligands. These ligands consist of a complex of sialomucins, candidates for which 
      are glycosylation- dependent cell adhesion molecule 1 (GlyCAM-1), CD34, and
      podocalyxin. The ligands must be sialylated, fucosylated, and sulfated for
      optimal recognition by L-selectin. Our previous structural characterization of
      GlyCAM-1 has demonstrated two sulfation modifications, Gal-6-sulfate and
      GlcNAc-6-sulfate in the context of sialyl Lewis x. We now report the cloning of a
      Gal-6-sulfotransferase and a GlcNAc-6-sulfotransferase, which can modify GlyCAM-1
      and CD34. The Gal-6-sulfotransferase shows a wide tissue distribution. In
      contrast, the GlcNAc-6-sulfotransferase is highly restricted to HEVs, as revealed
      by Northern analysis and in situ hybridization. Expression of either enzyme in
      Chinese hamster ovary cells, along with CD34 and fucosyltransferase VII, results 
      in ligand activity, as detected by binding of an L-selectin/IgM chimera. When
      coexpressed, the two sulfotransferases synergize to produce strongly enhanced
      chimera binding.
FAU - Bistrup, A
AU  - Bistrup A
AD  - Department of Anatomy and Program in Immunology, University of California, San
      Francisco, California 94143, USA.
FAU - Bhakta, S
AU  - Bhakta S
FAU - Lee, J K
AU  - Lee JK
FAU - Belov, Y Y
AU  - Belov YY
FAU - Gunn, M D
AU  - Gunn MD
FAU - Zuo, F R
AU  - Zuo FR
FAU - Huang, C C
AU  - Huang CC
FAU - Kannagi, R
AU  - Kannagi R
FAU - Rosen, S D
AU  - Rosen SD
FAU - Hemmerich, S
AU  - Hemmerich S
LA  - eng
SI  - GENBANK/AF131235
SI  - GENBANK/AF131236
GR  - R01 GM057411/GM/NIGMS NIH HHS/United States
GR  - R37 GM023547/GM/NIGMS NIH HHS/United States
GR  - GM57411/GM/NIGMS NIH HHS/United States
GR  - R37 GM23547/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (6'-sulfated sialyl Lewis x)
RN  - 0 (Carbohydrates)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Ligands)
RN  - 0 (Mucins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Sialyl Lewis X Antigen)
RN  - 126880-86-2 (L-Selectin)
RN  - 145895-89-2 (sulfated glycoprotein p50)
RN  - 70FD1KFU70 (Sulfur)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.- (carbohydrate sulfotransferases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Carbohydrate Sequence
MH  - Carbohydrates/genetics
MH  - Cells, Cultured
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - L-Selectin/*metabolism
MH  - Lewis X Antigen/analogs & derivatives
MH  - Ligands
MH  - Molecular Sequence Data
MH  - Mucins/metabolism
MH  - Oligosaccharides/metabolism
MH  - Sialyl Lewis X Antigen/analogs & derivatives
MH  - Sulfotransferases/chemistry/genetics/*metabolism
MH  - Sulfur/metabolism
PMC - PMC2133194
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - 10.1083/jcb.145.4.899 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 May 17;145(4):899-910. doi: 10.1083/jcb.145.4.899.