PMID- 10330412
OWN - NLM
STAT- MEDLINE
DCOM- 19990721
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 4
DP  - 1999 May 17
TI  - Neurotractin, a novel neurite outgrowth-promoting Ig-like protein that interacts 
      with CEPU-1 and LAMP.
PG  - 865-76
AB  - The formation of axon tracts in nervous system histogenesis is the result of
      selective axon fasciculation and specific growth cone guidance in embryonic
      development. One group of proteins implicated in neurite outgrowth,
      fasciculation, and guidance is the neural members of the Ig superfamily (IgSF).
      In an attempt to identify and characterize new proteins of this superfamily in
      the developing nervous system, we used a PCR-based strategy with degenerated
      primers that represent conserved sequences around the characteristic cysteine
      residues of Ig-like domains. Using this approach, we identified a novel neural
      IgSF member, termed neurotractin. This GPI-linked cell surface glycoprotein is
      composed of three Ig-like domains and belongs to the IgLON subgroup of neural
      IgSF members. It is expressed in two isoforms with apparent molecular masses of
      50 and 37 kD, termed L-form and S-form, respectively. Monoclonal antibodies were 
      used to analyze its biochemical features and histological distribution.
      Neurotractin is restricted to subsets of developing commissural and longitudinal 
      axon tracts in the chick central nervous system. Recombinant neurotractin
      promotes neurite outgrowth of telencephalic neurons and interacts with the IgSF
      members CEPU-1 (KD = 3 x 10(-8) M) and LAMP. Our data suggest that neurotractin
      participates in the regulation of neurite outgrowth in the developing brain.
FAU - Marg, A
AU  - Marg A
AD  - Max-Delbruck-Center for Molecular Medicine, D-13092 Berlin, Germany.
FAU - Sirim, P
AU  - Sirim P
FAU - Spaltmann, F
AU  - Spaltmann F
FAU - Plagge, A
AU  - Plagge A
FAU - Kauselmann, G
AU  - Kauselmann G
FAU - Buck, F
AU  - Buck F
FAU - Rathjen, F G
AU  - Rathjen FG
FAU - Brummendorf, T
AU  - Brummendorf T
LA  - eng
SI  - GENBANK/AJ132998
SI  - GENBANK/AJ132999
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Avian Proteins)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (DNA, Complementary)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (limbic system-associated membrane protein)
RN  - 0 (neural CEPU-1 protein, chicken)
RN  - 0 (neurotractin protein, chicken)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Avian Proteins
MH  - Axons/metabolism
MH  - Base Sequence
MH  - Brain/metabolism
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Adhesion Molecules, Neuronal/*metabolism
MH  - Central Nervous System
MH  - Chick Embryo
MH  - Chickens
MH  - Cricetinae
MH  - DNA, Complementary
MH  - GPI-Linked Proteins
MH  - Immunoglobulin G
MH  - Immunoglobulins/*metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Membrane Proteins/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurites/*metabolism
MH  - Neurons
MH  - Telencephalon
PMC - PMC2133198
EDAT- 1999/05/20 00:00
MHDA- 1999/05/20 00:01
CRDT- 1999/05/20 00:00
PHST- 1999/05/20 00:00 [pubmed]
PHST- 1999/05/20 00:01 [medline]
PHST- 1999/05/20 00:00 [entrez]
AID - 10.1083/jcb.145.4.865 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 May 17;145(4):865-76. doi: 10.1083/jcb.145.4.865.