PMID- 10330186
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 6
DP  - 1999 Jun
TI  - Cytoplasmic localization of human cdc25C during interphase requires an intact
      14-3-3 binding site.
PG  - 4465-79
AB  - cdc25C induces mitosis by activating the cdc2-cyclin B complex. The intracellular
      localization of cyclin B1 is regulated in a cell cycle-specific manner, and its
      entry into the nucleus may be required for the initiation of mitosis. To
      determine the cellular localization of cdc25C, monoclonal antibodies specific for
      cdc25C were developed and used to demonstrate that in human cells, cdc25C is
      retained in the cytoplasm during interphase. A deletion analysis identified a
      58-amino-acid region (amino acids 201 to 258) in cdc25C that was required for the
      cytoplasmic localization of cdc25C. This region contained a specific binding site
      for 14-3-3 proteins, and mutations in cdc25C that disrupted 14-3-3 binding also
      disrupted the cytoplasmic localization of cdc25C during interphase. cdc25C
      proteins that do not contain a binding site for 14-3-3 proteins showed a
      pancellular localization and an increased ability to induce premature chromosome 
      condensation. The cytoplasmic localization of cdc25C was not altered by gamma
      irradiation or treatment with the nuclear export inhibitor leptomycin B. These
      results suggest that 14-3-3 proteins may negatively regulate cdc25C function by
      sequestering cdc25C in the cytoplasm.
FAU - Dalal, S N
AU  - Dalal SN
AD  - Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts
      02115, USA.
FAU - Schweitzer, C M
AU  - Schweitzer CM
FAU - Gan, J
AU  - Gan J
FAU - DeCaprio, J A
AU  - DeCaprio JA
LA  - eng
GR  - P01 CA050661/CA/NCI NIH HHS/United States
GR  - R01 CA063113/CA/NCI NIH HHS/United States
GR  - CA-50661/CA/NCI NIH HHS/United States
GR  - CA-63113/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCNB1 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclin B1)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 500-44-7 (Mimosine)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.48 (CDC25C protein, human)
RN  - EC 3.1.3.48 (cdc25 Phosphatases)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Blotting, Western
MH  - Cell Cycle
MH  - Cell Cycle Proteins/*analysis/immunology/physiology
MH  - Cyclin B/metabolism
MH  - Cyclin B1
MH  - Cytoplasm/*chemistry
MH  - Fibroblasts/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - *Interphase
MH  - Mimosine/pharmacology
MH  - *Mitosis
MH  - Nuclear Localization Signals
MH  - Osteosarcoma/metabolism
MH  - Phosphoprotein Phosphatases/*analysis/immunology/physiology
MH  - Plasmids
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Recombinant Fusion Proteins
MH  - Subcellular Fractions
MH  - Time Factors
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - *cdc25 Phosphatases
PMC - PMC104405
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1128/mcb.19.6.4465 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jun;19(6):4465-79. doi: 10.1128/mcb.19.6.4465.