PMID- 10330166
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 6
DP  - 1999 Jun
TI  - RNA polymerase III transcription factor IIIB is a target for repression by pocket
      proteins p107 and p130.
PG  - 4255-61
AB  - RNA polymerase III (Pol III) transcription is subject to repression by the
      retinoblastoma protein RB, both in vitro and in vivo (R. J. White, D. Trouche, K.
      Martin, S. P. Jackson, and T. Kouzarides, Nature 382:88-90, 1996). This is
      achieved through a direct interaction between RB and TFIIIB, a multisubunit
      factor that is required for the expression of all Pol III templates (C. G. C.
      Larminie, C. A. Cairns, R. Mital, K. Martin, T. Kouzarides, S. P. Jackson, and R.
      J. White, EMBO J. 16:2061-2071, 1997; W.-M. Chu, Z. Wang, R. G. Roeder, and C. W.
      Schmid, J. Biol. Chem. 272:14755-14761, 1997). p107 and p130 are two closely
      related proteins that display 30 to 35% identity with the RB polypeptide and
      share some of its functions. We show that p107 and p130 can both repress Pol III 
      transcription in transient transfection assays or when added to cell extracts.
      Pull-down assays and immunoprecipitations using recombinant components
      demonstrate that a subunit of TFIIIB interacts physically with p107 and p130. In 
      addition, endogenous TFIIIB is shown by cofractionation and coimmunoprecipitation
      to associate stably with both p107 and p130. Disruption of this interaction in
      vivo by using the E7 oncoprotein of human papillomavirus results in a marked
      increase in Pol III transcription. Pol III activity is also deregulated in
      fibroblasts derived from p107 p130 double knockout mice. We conclude that TFIIIB 
      is targeted for repression not only by RB but also by its relatives p107 and
      p130.
FAU - Sutcliffe, J E
AU  - Sutcliffe JE
AD  - Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular
      Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
FAU - Cairns, C A
AU  - Cairns CA
FAU - McLees, A
AU  - McLees A
FAU - Allison, S J
AU  - Allison SJ
FAU - Tosh, K
AU  - Tosh K
FAU - White, R J
AU  - White RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (RBL1 protein, human)
RN  - 0 (RBL2 protein, human)
RN  - 0 (Rbl1 protein, mouse)
RN  - 0 (Rbl2 protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma-Like Protein p107)
RN  - 0 (Retinoblastoma-Like Protein p130)
RN  - 0 (Transcription Factor TFIIIB)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Chloramphenicol O-Acetyltransferase/metabolism
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Knockout
MH  - Nuclear Proteins/*genetics
MH  - Osteosarcoma/metabolism
MH  - Papillomaviridae/metabolism
MH  - Phosphoproteins/*genetics
MH  - Plasmids
MH  - Precipitin Tests
MH  - *Proteins
MH  - RNA Polymerase III/*genetics
MH  - Recombinant Fusion Proteins
MH  - Retinoblastoma-Like Protein p107
MH  - Retinoblastoma-Like Protein p130
MH  - Transcription Factor TFIIIB
MH  - Transcription Factors/*genetics
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
PMC - PMC104385
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1128/mcb.19.6.4255 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jun;19(6):4255-61. doi: 10.1128/mcb.19.6.4255.