PMID- 10330152
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 6
DP  - 1999 Jun
TI  - Transcriptional repressor ERF is a Ras/mitogen-activated protein kinase target
      that regulates cellular proliferation.
PG  - 4121-33
AB  - A limited number of transcription factors have been suggested to be regulated
      directly by Erks within the Ras/mitogen-activated protein kinase signaling
      pathway. In this paper we demonstrate that ERF, a ubiquitously expressed
      transcriptional repressor that belongs to the Ets family, is physically
      associated with and phosphorylated in vitro and in vivo by Erks. This
      phosphorylation determines the ERF subcellular localization. Upon mitogenic
      stimulation, ERF is immediately phosphorylated and exported to the cytoplasm. The
      export is blocked by specific Erk inhibitors and is abolished when residues
      undergoing phosphorylation are mutated to alanine. Upon growth factor
      deprivation, ERF is rapidly dephosphorylated and transported back into the
      nucleus. Phosphorylation-defective ERF mutations suppress Ras-induced
      tumorigenicity and arrest the cells at the G0/G1 phase of the cell cycle. Our
      findings strongly suggest that ERF may be important in the control of cellular
      proliferation during the G0/G1 transition and that it may be one of the effectors
      in the mammalian Ras signaling pathway.
FAU - Le Gallic, L
AU  - Le Gallic L
AD  - IMBB-FORTH, University of Crete, Voutes, Heraklion, Crete 714-09, Greece.
FAU - Sgouras, D
AU  - Sgouras D
FAU - Beal, G Jr
AU  - Beal G Jr
FAU - Mavrothalassitis, G
AU  - Mavrothalassitis G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERF protein, human)
RN  - 0 (ETS2 protein, human)
RN  - 0 (Ets2 protein, mouse)
RN  - 0 (Proto-Oncogene Protein c-ets-2)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism/*physiology
MH  - Cell Cycle
MH  - Cell Division/*physiology
MH  - Cell Line, Transformed
MH  - Chloramphenicol O-Acetyltransferase/metabolism
MH  - Chromatography, Gel
MH  - *DNA-Binding Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Immunoblotting
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mice
MH  - *Mitogen-Activated Protein Kinases
MH  - Models, Genetic
MH  - Mutagenesis
MH  - Phosphorylation
MH  - Proto-Oncogene Protein c-ets-2
MH  - Proto-Oncogene Proteins/metabolism/*physiology
MH  - *Repressor Proteins
MH  - Signal Transduction
MH  - Time Factors
MH  - Trans-Activators/metabolism/*physiology
MH  - *Transcription Factors
MH  - ras Proteins/*physiology
PMC - PMC104371
EDAT- 1999/05/18 05:26
MHDA- 2001/03/28 10:01
CRDT- 1999/05/18 05:26
PHST- 1999/05/18 05:26 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/05/18 05:26 [entrez]
AID - 10.1128/mcb.19.6.4121 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jun;19(6):4121-33. doi: 10.1128/mcb.19.6.4121.