PMID- 10329687
OWN - NLM
STAT- MEDLINE
DCOM- 19990709
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 21
DP  - 1999 May 21
TI  - Human placental Na+-dependent multivitamin transporter. Cloning, functional
      expression, gene structure, and chromosomal localization.
PG  - 14875-83
AB  - We have cloned the human Na+-dependent multivitamin transporter (SMVT), which
      transports the water-soluble vitamins pantothenate, biotin, and lipoate, from a
      placental choriocarcinoma cell line (JAR). The cDNA codes for a protein of 635
      amino acids with 12 transmembrane domains and 4 putative sites for N-linked
      glycosylation. The human SMVT exhibits a high degree of homology (84% identity
      and 89% similarity) to the rat counterpart. When expressed in HRPE cells, the
      cDNA-induced transport process is obligatorily dependent on Na+ and accepts
      pantothenate, biotin, and lipoate as substrates. The relationship between the
      cDNA-specific uptake rate of pantothenate or biotin and Na+ concentration is
      sigmoidal with a Na+:vitamin stoichiometry of 2:1. The human SMVT, when expressed
      in Xenopus laevis oocytes, induces inward currents in the presence of
      pantothenate, biotin, and lipoate in a Na+-, concentration-, and
      potential-dependent manner. We also report here on the structural organization
      and chromosomal localization of the human SMVT gene. The SMVT gene is
      approximately 14 kilobase pairs in length and consists of 17 exons. The SMVT gene
      is located on chromosome 2p23 as evidenced by somatic cell hybrid analysis and
      fluorescence in situ hybridization.
FAU - Wang, H
AU  - Wang H
AD  - Department of Biochemistry & Molecular Biology, Medical College of Georgia,
      Augusta, Georgia 30912, USA.
FAU - Huang, W
AU  - Huang W
FAU - Fei, Y J
AU  - Fei YJ
FAU - Xia, H
AU  - Xia H
FAU - Yang-Feng, T L
AU  - Yang-Feng TL
FAU - Leibach, F H
AU  - Leibach FH
FAU - Devoe, L D
AU  - Devoe LD
FAU - Ganapathy, V
AU  - Ganapathy V
FAU - Prasad, P D
AU  - Prasad PD
LA  - eng
SI  - GENBANK/AF069307
SI  - GENBANK/AF116241
GR  - HD 33347/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Symporters)
RN  - 0 (biotin transporter)
RN  - 19F5HK2737 (Pantothenic Acid)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport, Active
MH  - Biotin/metabolism
MH  - Carrier Proteins/biosynthesis/*genetics/isolation & purification
MH  - Choriocarcinoma
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Humans
MH  - Membrane Glycoproteins/biosynthesis/*genetics/isolation & purification
MH  - Molecular Sequence Data
MH  - Pantothenic Acid/metabolism
MH  - Placenta
MH  - *Symporters
MH  - Tumor Cells, Cultured
MH  - Uterine Neoplasms
MH  - Xenopus laevis
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1074/jbc.274.21.14875 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 21;274(21):14875-83. doi: 10.1074/jbc.274.21.14875.