PMID- 10329666
OWN - NLM
STAT- MEDLINE
DCOM- 19990709
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 21
DP  - 1999 May 21
TI  - The ankyrin repeat-containing adaptor protein Tvl-1 is a novel substrate and
      regulator of Raf-1.
PG  - 14706-15
AB  - Tvl-1 is a 269-amino acid ankyrin repeat protein expressed primarily in thymus,
      lung, and testes that was identified by screening a murine T-cell two-hybrid cDNA
      library for proteins that associate with the serine-threonine kinase Raf-1. The
      interaction of Tvl-1 with Raf-1 was confirmed by co-immunoprecipitation of the
      two proteins from COS-1 cells transiently transfected with Tvl-1 and Raf-1
      expression constructs as well as by co-immunoprecipitation of the endogenous
      proteins from CV-1 and NB2 cells. Tvl-1 interacts with Raf-1 via its
      carboxyl-terminal ankyrin repeat domain. The same domain also mediates Tvl-1
      homodimerization. Tvl-1 was detected by immunofluorescence in both the cytoplasm 
      and the nucleus suggesting that in addition to Raf-1 it may also interact with
      nuclear proteins. Activated Raf-1 phosphorylates Tvl-1 both in vitro and in vivo.
      In baculovirus-infected Sf9 insect cells, Tvl-1 potentiates the activation of
      Raf-1 by Src and Ras while in COS-1 cells it potentiates the activation of Raf-1 
      by EGF. These data suggest that Tvl-1 is both a target as well as a regulator of 
      Raf-1. The human homologue of Tvl-1 maps to chromosome 19p12, upstream of MEF2B
      with the two genes in a head to head arrangement.
FAU - Lin, J H
AU  - Lin JH
AD  - Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania
      19107, USA.
FAU - Makris, A
AU  - Makris A
FAU - McMahon, C
AU  - McMahon C
FAU - Bear, S E
AU  - Bear SE
FAU - Patriotis, C
AU  - Patriotis C
FAU - Prasad, V R
AU  - Prasad VR
FAU - Brent, R
AU  - Brent R
FAU - Golemis, E A
AU  - Golemis EA
FAU - Tsichlis, P N
AU  - Tsichlis PN
LA  - eng
SI  - GENBANK/AF123704
GR  - CA06927/CA/NCI NIH HHS/United States
GR  - R01-CA38147/CA/NCI NIH HHS/United States
GR  - T32-CA09683/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Rfxank protein, mouse)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Ankyrin Repeat
MH  - Carrier Proteins/isolation & purification/*physiology
MH  - Cells, Cultured
MH  - DNA-Binding Proteins
MH  - Molecular Sequence Data
MH  - Proto-Oncogene Proteins c-raf/*physiology
MH  - Substrate Specificity
MH  - Transcription Factors
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1074/jbc.274.21.14706 [doi]
AID - S0021-9258(19)73147-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 May 21;274(21):14706-15. doi: 10.1074/jbc.274.21.14706.