PMID- 10329627
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20191217
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 10
DP  - 1999 May 17
TI  - Transcriptional cofactors of the FOG family interact with GATA proteins by means 
      of multiple zinc fingers.
PG  - 2812-22
AB  - Friend of GATA-1 (FOG-1) is a zinc finger protein that has been shown to interact
      physically with the erythroid DNA-binding protein GATA-1 and modulate its
      transcriptional activity. Recently, two new members of the FOG family have been
      identified: a mammalian protein, FOG-2, that also associates with GATA-1 and
      other mammalian GATA factors; and U-shaped, a Drosophila protein that interacts
      with the Drosophila GATA protein Pannier. FOG proteins contain multiple zinc
      fingers and it has been shown previously that the sixth finger of FOG-1 interacts
      specifically with the N-finger but not the C-finger of GATA-1. Here we show that 
      fingers 1, 5 and 9 of FOG-1 also interact with the N-finger of GATA-1 and that
      FOG-2 and U-shaped also contain multiple GATA-interacting fingers. We define the 
      key contact residues and show that these residues are highly conserved in
      GATA-interacting fingers. We examine the effect of selectively mutating the four 
      interacting fingers of FOG-1 and show that each contributes to FOG-1's ability to
      modulate GATA-1 activity. Finally, we show that FOG-1 can repress GATA-1-mediated
      activation and present evidence that this ability involves the recently described
      CtBP co-repressor proteins that recognize all known FOG proteins.
FAU - Fox, A H
AU  - Fox AH
AD  - Department of Biochemistry, G08, University of Sydney, NSW, 2006, Australia.
FAU - Liew, C
AU  - Liew C
FAU - Holmes, M
AU  - Holmes M
FAU - Kowalski, K
AU  - Kowalski K
FAU - Mackay, J
AU  - Mackay J
FAU - Crossley, M
AU  - Crossley M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (GATA1 protein, human)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZFPM1 protein, human)
RN  - 0 (ZFPM2 protein, human)
RN  - 0 (Zfpm1 protein, mouse)
RN  - 0 (Zfpm2 protein, mouse)
RN  - EC 1.1.- (Ctbp2 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics/metabolism
MH  - Conserved Sequence
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - GATA1 Transcription Factor
MH  - Genes, Reporter
MH  - Humans
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Phosphoproteins/genetics
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Repressor Proteins/genetics
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics/metabolism
MH  - Yeasts
MH  - Zinc Fingers/*genetics
PMC - PMC1171362
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1093/emboj/18.10.2812 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 17;18(10):2812-22. doi: 10.1093/emboj/18.10.2812.