PMID- 10329441
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 3
DP  - 1999 May 19
TI  - Interaction cloning and characterization of the cDNA encoding the human
      prenylated rab acceptor (PRA1).
PG  - 657-62
AB  - Rab proteins are small GTPases involved in the regulation of intracellular
      membrane traffic in mammalian cells. In order to find Rab-interacting proteins we
      performed a two-hybrid screening using a human brain cDNA library. Here we report
      the isolation of a full-length human cDNA clone coding for a protein of 185 amino
      acids. This protein interacts strongly with the Rab4b, Rab5a, and Rab5c proteins 
      and weakly with Rab4a, Rab6, Rab7, Rab17, and Rab22 in the two-hybrid assay.
      Comparison with the Data Bank revealed that this clone represents the human
      homolog of the previously isolated rat Prenylated Rab Acceptor (rPRA1). Analysis 
      of mRNA expression shows a single abundant mRNA of about 0.8 kb ubiquitously
      expressed. Western blot analysis of the overexpressed protein shows a band of the
      expected size equally distributed between cytosol and membranes.
CI  - Copyright 1999 Academic Press.
FAU - Bucci, C
AU  - Bucci C
AD  - Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano" and
      Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle 
      Ricerche, Universita degli Studi di Napoli "Federico II", Via S. Pansini 5,
      Napoli, Italy.
FAU - Chiariello, M
AU  - Chiariello M
FAU - Lattero, D
AU  - Lattero D
FAU - Maiorano, M
AU  - Maiorano M
FAU - Bruni, C B
AU  - Bruni CB
LA  - eng
SI  - GENBANK/AJ133534
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Rabac1 protein, rat)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.1.- (RABAC1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*genetics/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - GTP-Binding Proteins
MH  - Humans
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Vesicular Transport Proteins
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0006-291X(99)90651-4 [pii]
AID - 10.1006/bbrc.1999.0651 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 May 19;258(3):657-62. doi:
      10.1006/bbrc.1999.0651.