PMID- 10329428
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20051117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 3
DP  - 1999 May 19
TI  - Cloning and functional characterization of a sodium-dependent phosphate
      transporter expressed in human lung and small intestine.
PG  - 578-82
AB  - A cDNA clone with 53% amino acid identity to the human type II sodium-dependent
      phosphate transporter (NaPi-3) was isolated from human small intestine and lung. 
      Functional characterization in Xenopus laevis oocytes showed this cDNA to encode 
      a sodium-dependent phosphate transporter. The electrogenic response is similar to
      that found in other type II transporters but an inverse pH dependence was
      observed. By Northern blot, a 4.2-kb transcript was found to be abundantly
      expressed in lung and, to a lesser degree, in several other tissues of epithelial
      origin including small intestine, pancreas, prostate, and kidney. This transcript
      encompasses a 2.073-kb open reading frame which is most closely related (78%
      amino acid identity) to the mouse sodium-dependent phosphate transporter IIb
      isoform. This novel transporter, designated human NaPi-3b (Genbank AF111856),
      appears to be an isoform of the mammalian renal type II co-transporter family.
CI  - Copyright 1999 Academic Press.
FAU - Feild, J A
AU  - Feild JA
AD  - Department of Molecular Biology, Department of Renal Pharmacology, Smithkline
      Beecham Pharmaceuticals, 709 Swedeland Road, King of Prussia, Pennsylvania,
      19406, USA. John_A_Field@sbphrd.com
FAU - Zhang, L
AU  - Zhang L
FAU - Brun, K A
AU  - Brun KA
FAU - Brooks, D P
AU  - Brooks DP
FAU - Edwards, R M
AU  - Edwards RM
LA  - eng
SI  - GENBANK/AF111856
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Sodium-Phosphate Cotransporter Proteins)
RN  - 0 (Sodium-Phosphate Cotransporter Proteins, Type II)
RN  - 0 (Symporters)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Intestine, Small/*metabolism
MH  - Lung/*metabolism
MH  - Molecular Sequence Data
MH  - Oocytes/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Sodium-Phosphate Cotransporter Proteins
MH  - Sodium-Phosphate Cotransporter Proteins, Type II
MH  - *Symporters
MH  - Xenopus
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0006-291X(99)90666-6 [pii]
AID - 10.1006/bbrc.1999.0666 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 May 19;258(3):578-82. doi:
      10.1006/bbrc.1999.0666.