PMID- 10329418
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 3
DP  - 1999 May 19
TI  - Carboxy-terminally truncated form of a coactivator UTF1 stimulates transcription 
      from a variety of gene promoters through the TATA Box.
PG  - 519-23
AB  - We have recently isolated a novel transcriptional coactivator, UTF1, which is
      expressed mainly in pluripotent embryonic stem cells (Okuda, A., Fukushima, A.,
      Nishimoto, M., Orimo, A., Yamagishi, T., Nabeshima, Y., Kuro-o, M., Nabeshima,
      Y., Boon, K., Keaveney, M., Stunnenberg, H. G., and Muramatsu, M. EMBO J. 17,
      2019-2032, 1998). The UTF1 does not activate transcription nonspecifically, but
      boosts the level of transcription strictly in a specific upstream factor, ATF-2, 
      dependent manner in mammalian cells. However, when expressed in yeast cells, the 
      UTF1 displays a distinct activity, being able to augment the activity of minimal 
      promoter bearing only the TATA element. Thus, these results indicate that certain
      domains of UTF1 render the factor inactive in terms of stimulating transcription 
      through the basal transcription machinery in the absence of promoter-bound ATF-2 
      in mammalian cells. Here we report that the region bearing the leucine zipper
      motif is responsible for such biochemical properties of the UTF1. Indeed, UTF1
      lacking functional leucine zipper is able to rather promiscuously stimulate
      transcription from a number of basal gene promoters such as those of hsp70 and
      E1B genes in mammalian cells. We have also shown that this activation is executed
      through TATA box by the experiments using a TBP allele with an altered
      TATA-binding specificity. Moreover, we have found that Dr1-mediated repression of
      transcription can be overcome by expression of this mutant UTF1, indicating that 
      the observed stimulation of transcription is at least in part due to its action
      as an anti-repressor.
CI  - Copyright 1999 Academic Press.
FAU - Fukushima, A
AU  - Fukushima A
AD  - Department of Biochemistry, Saitama Medical School, 38 Morohongo Moroyama
      Iruma-gun, Saitama, 350-0495, Japan.
FAU - Nishimoto, M
AU  - Nishimoto M
FAU - Okuda, A
AU  - Okuda A
FAU - Muramatsu, M
AU  - Muramatsu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adenovirus E1B Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Adenovirus E1B Proteins/genetics
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA-Binding Proteins/genetics
MH  - Glutathione Transferase/genetics
MH  - HSP70 Heat-Shock Proteins/genetics
MH  - Mammals
MH  - Mutagenesis, Site-Directed
MH  - *Promoter Regions, Genetic
MH  - *TATA Box
MH  - TATA-Box Binding Protein
MH  - Trans-Activators/chemistry/*metabolism
MH  - Transcription Factors/genetics
MH  - *Transcription, Genetic
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0006-291X(99)90673-3 [pii]
AID - 10.1006/bbrc.1999.0673 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 May 19;258(3):519-23. doi:
      10.1006/bbrc.1999.0673.