PMID- 10329386
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20071114
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 2
DP  - 1999 May 10
TI  - The novel Mrf-2 DNA-binding domain recognizes a five-base core sequence through
      major and minor-groove contacts.
PG  - 326-31
AB  - Recent NMR studies of the purified Mrf-2 DNA-binding domain peptide have shown
      that its structure differs significantly from previously characterized classes of
      DNA-binding domains. Here we report biochemical studies of the DNA-binding
      properties of this peptide. Binding interference and binding site selection
      assays indicated that Mrf-2 requires the core sequence AATA(C/T) for high
      affinity binding. Kinetic analyses of several selected sequences indicated that
      the core sequence alone is not sufficient for high affinity binding, however.
      Kinetic analyses were also performed using a series of synthetic oligonucleotides
      with single base analogues at each position in the core sequence. Base analogues 
      that altered the major groove structure reduced or eliminated Mrf-2 binding when 
      present in the second, third, and fourth base-pairs of the core sequence, but had
      little or no effect in the first and fifth positions. These results suggest that 
      Mrf-2 contacts both the major and minor grooves of its target sequences.
CI  - Copyright 1999 Academic Press.
FAU - Whitson, R H
AU  - Whitson RH
AD  - Department of Molecular Biology, Beckman Research Institute of the City of Hope, 
      1450 East Duarte Road, Duarte, California 91010, USA.
FAU - Huang, T
AU  - Huang T
FAU - Itakura, K
AU  - Itakura K
LA  - eng
GR  - GM54190/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Myogenic Regulatory Factors)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - DNA Primers
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - Kinetics
MH  - Myogenic Regulatory Factors/chemistry/*metabolism
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0006-291X(99)90643-5 [pii]
AID - 10.1006/bbrc.1999.0643 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 May 10;258(2):326-31. doi:
      10.1006/bbrc.1999.0643.