PMID- 10329382
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 2
DP  - 1999 May 10
TI  - Phosphorylation of the cytoplasmic domain of Alzheimer's beta-amyloid precursor
      protein at Ser655 by a novel protein kinase.
PG  - 300-5
AB  - The cytoplasmic domain of Alzheimer's beta-amyloid precursor protein (APP) can be
      phosphorylated at Thr654, Ser655, and Thr668 (APP695 isoform numbering). Previous
      studies demonstrated that Ser655 of APP was phosphorylated by protein kinase C
      (PKC) and calmodulin-dependent protein kinase II (CaMKII) in vitro and by
      unidentified protein kinase(s) in vivo. We report here the characterization of a 
      novel protein kinase (designated APP kinase I) which phosphorylates Ser655 of
      APP. APP kinase I was partially purified over 7,000-fold from rat brain and
      identified as a approximately 43 kDa protein that is distinct from a number of
      known protein kinases, including PKC and extracellular signal-regulated kinases
      (ERKs). The identification of a novel protein kinase that phosphorylates Ser655
      will hopefully contribute to our understanding of the metabolism and/or function 
      of APP in the pathogenesis of Alzheimer's disease (AD).
CI  - Copyright 1999 Academic Press.
FAU - Isohara, T
AU  - Isohara T
AD  - Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New
      York, New York 10021-6399, USA.
FAU - Horiuchi, A
AU  - Horiuchi A
FAU - Watanabe, T
AU  - Watanabe T
FAU - Ando, K
AU  - Ando K
FAU - Czernik, A J
AU  - Czernik AJ
FAU - Uno, I
AU  - Uno I
FAU - Greengard, P
AU  - Greengard P
FAU - Nairn, A C
AU  - Nairn AC
FAU - Suzuki, T
AU  - Suzuki T
LA  - eng
GR  - AG09464/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (beta-amyloid precursor protein kinase I)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Alzheimer Disease/enzymology/metabolism
MH  - Amyloid beta-Protein Precursor/chemistry/*metabolism
MH  - Animals
MH  - Brain/enzymology
MH  - Chromatography, Ion Exchange
MH  - Cytoplasm/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Rats
MH  - Serine/*metabolism
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0006-291X(99)90637-X [pii]
AID - 10.1006/bbrc.1999.0637 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 May 10;258(2):300-5. doi:
      10.1006/bbrc.1999.0637.