PMID- 10329126
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20120620
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 288
IP  - 1
DP  - 1999 Apr 23
TI  - Role of the human and murine cyclin T proteins in regulating HIV-1
      tat-activation.
PG  - 57-69
AB  - Human cyclin T1 markedly stimulates tat-activation in rodent cells which are
      normally poorly responsive to the effects of Tat. This result suggests that there
      are likely to be critical differences in the murine and human cyclin T1 proteins.
      Here, we analyzed the role of the murine and human cyclin T1 proteins in addition
      to the human cyclin T2a and T2b proteins on regulating tat-activation. Only the
      human cyclin T1 protein efficiently formed a complex with Tat bound to TAR RNA.
      This difference in function was due to the presence of a cysteine residue in
      human cyclin T1 at position 261 rather than a tyrosine or asparagine residue
      which are found in the murine cyclin T1 protein and the human cyclin T2a and T2b 
      proteins, respectively. A mouse cyclin T1 protein containing a substitution of
      tyrosine residue 261 with a cysteine residue, was able to interact with Tat and
      stimulate tat-transactivation in rodent cells. Likewise, substitution of a
      cysteine residue for an asparagine residue at position 260 of the cyclin T2a and 
      T2b proteins also resulted in their ability to interact with Tat and stimulate
      tat-activation in rodent cells. The data indicate that a specific residue in the 
      cyclin T proteins is required for their in vitro interaction with Tat and their
      ability to stimulate in vivo tat-activation.
CI  - Copyright 1999 Academic Press.
FAU - Kwak, Y T
AU  - Kwak YT
AD  - Division of Hematology-Oncology, Department of Medicine, Harold Simmons Cancer
      Center, University of Texas Southwestern Medical Center, Dallas, TX, 75235-8594, 
      USA.
FAU - Ivanov, D
AU  - Ivanov D
FAU - Guo, J
AU  - Guo J
FAU - Nee, E
AU  - Nee E
FAU - Gaynor, R B
AU  - Gaynor RB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (CCNT1 protein, human)
RN  - 0 (Ccnt1 protein, mouse)
RN  - 0 (Cyclin T)
RN  - 0 (Cyclins)
RN  - 0 (Gene Products, tat)
RN  - 0 (Macromolecular Substances)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.22 (CDK9 protein, human)
RN  - EC 2.7.11.22 (Cdk9 protein, mouse)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 9)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Cyclin T
MH  - Cyclin-Dependent Kinase 9
MH  - Cyclin-Dependent Kinases/metabolism
MH  - Cyclins/*chemistry/metabolism
MH  - *Gene Expression Regulation, Viral
MH  - Gene Products, tat/*metabolism
MH  - Genes, Reporter
MH  - HIV Long Terminal Repeat/*genetics
MH  - HIV-1/*genetics
MH  - HeLa Cells
MH  - Humans
MH  - Luciferases/biosynthesis/genetics
MH  - Macromolecular Substances
MH  - Mice
MH  - *Protein Structure, Tertiary
MH  - RNA Polymerase II/metabolism
MH  - RNA, Viral/genetics/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Deletion
MH  - *Transcriptional Activation
MH  - Transfection
MH  - tat Gene Products, Human Immunodeficiency Virus
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0022-2836(99)92664-5 [pii]
AID - 10.1006/jmbi.1999.2664 [doi]
PST - ppublish
SO  - J Mol Biol. 1999 Apr 23;288(1):57-69. doi: 10.1006/jmbi.1999.2664.