PMID- 10329026
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20071114
IS  - 1096-7192 (Print)
IS  - 1096-7192 (Linking)
VI  - 67
IP  - 1
DP  - 1999 May
TI  - Cloning and characterization of retinol dehydrogenase transcripts expressed in
      human epidermal keratinocytes.
PG  - 62-73
AB  - The normal growth and differentiation of the epidermis require an adequate supply
      of vitamin A. The active form of vitamin A for normal epidermal homeostasis is
      retinoic acid (RA). Retinoic acid controls the expression of retinoid-responsive 
      genes via interactions of the retinoic acid/nuclear receptor complexes at
      specific DNA sequences in their control regions. The message conveyed by RA is
      likely modulated by the concentration of the ligand available for binding to the 
      receptors. Following the uptake of plasma retinol, epidermal keratinocytes
      synthesize retinoic acid via two sequential reactions with retinaldehyde as an
      intermediate. Several retinol dehydrogenase (RDH) enzymes, members of the
      short-chain dehydrogenase/reductase (SDR) gene superfamily, catalyze the first
      and rate-limiting step that generates retinaldehyde from retinol bound to
      cellular retinol-binding protein (holo-CRBP). However, little is known about
      these enzymes and their genes in the epidermal cells. Our work describes the
      first member of the RDH family found in epidermis. We show that this gene is
      expressed predominantly in the differentiating spinous layers and that it is
      under positive, feed-forward regulation by retinoic acid. It encodes a protein
      that, using NAD+ as a preferred cofactor, utilizes free and CRBP-bound
      all-trans-retinol and steroids as substrates.
CI  - Copyright 1999 Academic Press.
FAU - Jurukovski, V
AU  - Jurukovski V
AD  - Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook,
      New York 11794-8702, USA.
FAU - Markova, N G
AU  - Markova NG
FAU - Karaman-Jurukovska, N
AU  - Karaman-Jurukovska N
FAU - Randolph, R K
AU  - Randolph RK
FAU - Su, J
AU  - Su J
FAU - Napoli, J L
AU  - Napoli JL
FAU - Simon, M
AU  - Simon M
LA  - eng
SI  - GENBANK/AF086735
GR  - DK36870/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Genet Metab
JT  - Molecular genetics and metabolism
JID - 9805456
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 11103-57-4 (Vitamin A)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.105 (retinol dehydrogenase)
SB  - IM
MH  - Alcohol Oxidoreductases/*genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Fractionation
MH  - Cells, Cultured
MH  - *Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary/isolation & purification
MH  - Dose-Response Relationship, Drug
MH  - Epidermis/anatomy & histology/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Keratinocytes/*metabolism
MH  - Liver/metabolism
MH  - Microsomes/metabolism
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tissue Distribution
MH  - Transfection
MH  - Vitamin A/metabolism
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S1096-7192(99)92840-8 [pii]
AID - 10.1006/mgme.1999.2840 [doi]
PST - ppublish
SO  - Mol Genet Metab. 1999 May;67(1):62-73. doi: 10.1006/mgme.1999.2840.