PMID- 10328927
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20111117
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 209
IP  - 2
DP  - 1999 May 15
TI  - Isolation of novel cDNAs by subtractions between the anterior mesendoderm of
      single mouse gastrula stage embryos.
PG  - 369-80
AB  - The anterior mesendoderm of mid- to late primitive streak stage mouse embryos has
      the ability to induce anterior neuroectodermal fate in naive epiblast [S.-L. Ang 
      and J. Rossant (1993) Development 118, 139-149]. A number of genes have been
      found to be expressed in this tissue, notably the transcription factor Lim1.
      Lim1-null mice have anterior mesendoderm defects that result in a lack of head
      formation. Thus, the anterior mesendoderm of gastrula stage mouse embryos should 
      express Lim1-regulated genes that are essential for head development. To identify
      Lim1-regulated genes, a differential screen with subtraction was developed, using
      cDNA pools that were amplified from the anterior mesendoderm of single wild-type 
      and Lim1-null gastrula stage embryos. This novel screen strategy has yielded 22
      cDNAs that show differential expression between anterior mesendoderm cells of
      wild-type and Lim1-null embryos. The expression of one novel cDNA SII6 initially 
      colocalizes with Lim1 in the anterior mesendoderm of gastrula stage embryos.
      Moreover, SII6 expression is undetectable in the anterior mesendoderm of
      Lim1-null embryos. This screen identifies a set of putative Lim1 target genes
      that may have important roles in vertebrate head formation. Furthermore, this
      differential screen strategy should provide a broadly applicable approach to
      identify differences in gene expression between embryonic tissues of limiting
      quantity.
CI  - Copyright 1999 Academic Press.
FAU - Shimono, A
AU  - Shimono A
AD  - Department of Molecular Genetics, University of Texas M. D. Anderson Cancer
      Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, USA.
FAU - Behringer, R R
AU  - Behringer RR
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (DNA, Complementary)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LIM-Homeodomain Proteins)
RN  - 0 (Lhx1 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - DNA, Complementary/genetics/*isolation & purification
MH  - Embryonic Induction
MH  - Embryonic and Fetal Development/*genetics
MH  - Endoderm/*chemistry/cytology
MH  - Gastrula/*chemistry/cytology
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Gestational Age
MH  - Homeodomain Proteins/physiology
MH  - LIM-Homeodomain Proteins
MH  - Mesoderm/*chemistry/cytology
MH  - Mice
MH  - Mice, Knockout
MH  - Subtraction Technique
MH  - Transcription Factors
MH  - Transcription, Genetic
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - S0012-1606(99)99256-4 [pii]
AID - 10.1006/dbio.1999.9256 [doi]
PST - ppublish
SO  - Dev Biol. 1999 May 15;209(2):369-80. doi: 10.1006/dbio.1999.9256.