PMID- 10327653
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20091119
IS  - 0022-104X (Print)
IS  - 0022-104X (Linking)
VI  - 285
IP  - 1
DP  - 1999 Apr 15
TI  - Phylogenetically conserved CK-II phosphorylation site of the murine homeodomain
      protein Hoxb-6.
PG  - 76-84
AB  - In an effort to characterize the signal transduction mechanisms that operate to
      regulate homeodomain protein function, we have analyzed the phosphorylation state
      of two homeodomain proteins, Hoxb-6 and Hoxc-8, in vitro and in vivo. The
      baculovirus expression system was employed to demonstrate that Hoxb-6 is
      phosphorylated in Sf9 cells while Hoxc-8 is not. Using two-dimensional tryptic
      phosphopeptide mapping and purified protein kinases, we demonstrate that Hoxb-6
      is phosphorylated in vitro by casein kinase II and cAMP-dependent protein kinase.
      The casein kinase II phosphorylation site was mapped to serine-214.
      Two-dimensional tryptic phosphopeptide mapping of immunoprecipitated Hoxb-6 from 
      mouse embryonic spinal cords demonstrates that the same peptide phosphorylated in
      vitro and in Sf9 cells by casein kinase II is also phosphorylated in vivo. The
      conservation of this site in several homeodomain proteins from various species is
      discussed.
FAU - Fienberg, A A
AU  - Fienberg AA
AD  - Department of Genetics, Yale University School of Medicine, Yale University, New 
      Haven, Connecticut 06510, USA. fienba@rockvax.rockefeller.edu
FAU - Nordstedt, C
AU  - Nordstedt C
FAU - Belting, H G
AU  - Belting HG
FAU - Czernik, A J
AU  - Czernik AJ
FAU - Nairn, A C
AU  - Nairn AC
FAU - Gandy, S
AU  - Gandy S
FAU - Greengard, P
AU  - Greengard P
FAU - Ruddle, F H
AU  - Ruddle FH
LA  - eng
GR  - AG09464 C/AG/NIA NIH HHS/United States
GR  - GM09966/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Zool
JT  - The Journal of experimental zoology
JID - 0375365
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Hoxb6 protein, mouse)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Casein Kinase II
MH  - Conserved Sequence
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Homeodomain Proteins/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Phylogeny
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Spodoptera
EDAT- 1999/05/18 05:24
MHDA- 2000/06/20 09:00
CRDT- 1999/05/18 05:24
PHST- 1999/05/18 05:24 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/18 05:24 [entrez]
AID - 10.1002/(SICI)1097-010X(19990415)285:1<76::AID-JEZ9>3.0.CO;2-K [pii]
PST - ppublish
SO  - J Exp Zool. 1999 Apr 15;285(1):76-84.