PMID- 10327052
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 14
DP  - 1999 Apr 8
TI  - Metastasis-association of the rat ortholog of the human epithelial glycoprotein
      antigen EGP314.
PG  - 2323-34
AB  - Screening for surface molecules expressed by metastasizing rat tumors had
      revealed evidence for metastasis-association of a molecule also expressed on
      epithelial cells. The similarity to the expression profile of the panepithelial
      glycoprotein EGP314 prompted us to isolate and sequence the gene and to explore
      functional features of the molecule in transfected tumor lines. The molecule
      D5.7A, named according to the antibody, D5.7, used for selection, indeed, is the 
      ortholog of EGP314 with 92% and 80% identity to the murine and the human
      molecules. Like EGP314, D5.7A has a particular cleavage site, a small cleavage
      product being resolved under reducing conditions from the membrane anchored part 
      of the molecule. Transfection of a low metastasizing fibrosarcoma,
      pheochromoblastoma and adenocarcinoma revealed that expression of D5.7A
      facilitates tumor progression. Depending on the origin of the tumor, D5.7A
      transfectants either metastasized via the lymphatic system (pheochromoblastoma,
      adenocarcinoma) or hematogeneously (fibrosarcoma). Particularly after proteolytic
      cleavage, D5.7A facilitated cell - cell adhesion and provided a proliferative
      signal upon crosslinking. Thus, the rat ortholog of EGP314 is involved in
      metastasis formation. Importantly, its functional activities apparently rely on
      proteolytic cleavage. These findings provide a first evidence on how a
      panepithelial marker can be involved in tumor progression.
FAU - Wurfel, J
AU  - Wurfel J
AD  - Department of Tumor Progression and Immune Defense, German Cancer Research
      Center, Heidelberg.
FAU - Rosel, M
AU  - Rosel M
FAU - Seiter, S
AU  - Seiter S
FAU - Claas, C
AU  - Claas C
FAU - Herlevsen, M
AU  - Herlevsen M
FAU - Weth, R
AU  - Weth R
FAU - Zoller, M
AU  - Zoller M
LA  - eng
SI  - GENBANK/AJ001044
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA, Complementary)
RN  - 0 (EGP314 antigen)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm/genetics/*physiology
MH  - Base Sequence
MH  - COS Cells
MH  - Cell Adhesion
MH  - Cell Aggregation
MH  - Cell Division
MH  - Cell Movement
MH  - Cloning, Molecular
MH  - Colonic Neoplasms/*genetics/pathology
MH  - DNA, Complementary/genetics
MH  - Fibrosarcoma/genetics/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - *Genes
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neoplasm Metastasis/*genetics
MH  - Neoplasm Proteins/genetics/*physiology
MH  - Oxidation-Reduction
MH  - Pancreatic Neoplasms/genetics/pathology
MH  - Pheochromocytoma/genetics/pathology
MH  - Rats/*genetics
MH  - Sequence Alignment
MH  - Sequence Homology
MH  - Species Specificity
MH  - Specific Pathogen-Free Organisms
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1038/sj.onc.1202542 [doi]
PST - ppublish
SO  - Oncogene. 1999 Apr 8;18(14):2323-34. doi: 10.1038/sj.onc.1202542.