PMID- 10327051
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 14
DP  - 1999 Apr 8
TI  - The N-terminal transactivation domain of ATF2 is a target for the co-operative
      activation of the c-jun promoter by p300 and 12S E1A.
PG  - 2311-21
AB  - The adenovirus E1A proteins activate the c-jun promoter through two
      Jun/ATF-binding sites, jun1 and jun2. P300, a transcriptional coactivator of
      several AP1 and ATF transcription factors has been postulated to play a role in
      this activation. Here, we present evidence that p300 can control c-jun
      transcription by acting as a cofactor for ATF2: (1) Over-expression of p300 was
      found to stimulate c-jun transcription both in the presence and absence of E1A.
      (2) Like E1A, p300 activates the c-jun promoter through the junl and jun2
      elements and preferentially activates the N-terminal domain of ATF2. (3)
      Co-immunoprecipitation assays of crude cell extracts indicate that endogenous
      p300/CBP(-like) proteins and ATF2 proteins are present in a multiprotein complex 
      that can bind specifically to the jun2 element. We further demonstrate that the
      Stress-Activated-Protein-Kinase (SAPK) target sites of ATF2, Thr69 and Thr71 are 
      not required for the formation of the p300/CBP-ATF2 multiprotein complex. These
      data indicate that E1A does not inhibit all transcription activation functions of
      p300, and, in fact, cooperates with p300 in the activation of the ATF2
      N-terminus.
FAU - Duyndam, M C
AU  - Duyndam MC
AD  - Laboratory for Molecular Carcinogenesis, Leiden University Medical Center, The
      Netherlands.
FAU - van Dam, H
AU  - van Dam H
FAU - Smits, P H
AU  - Smits PH
FAU - Verlaan, M
AU  - Verlaan M
FAU - van der Eb, A J
AU  - van der Eb AJ
FAU - Zantema, A
AU  - Zantema A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Adenovirus E1A Proteins)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 1114-81-4 (Phosphothreonine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (Mitogen-Activated Protein Kinase 12)
RN  - EC 2.7.1.24 (Mitogen-Activated Protein Kinase 9)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Activating Transcription Factor 2
MH  - Adenovirus E1A Proteins/*physiology
MH  - Animals
MH  - Binding Sites
MH  - Calcium-Calmodulin-Dependent Protein Kinases/physiology
MH  - Cell Line, Transformed
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - *Gene Expression Regulation, Viral
MH  - *Genes, jun
MH  - Humans
MH  - Macromolecular Substances
MH  - Mitogen-Activated Protein Kinase 12
MH  - Mitogen-Activated Protein Kinase 9
MH  - *Mitogen-Activated Protein Kinases
MH  - Multiprotein Complexes
MH  - Nuclear Proteins/genetics/*physiology
MH  - Phosphothreonine/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Kinases/physiology
MH  - Protein Processing, Post-Translational
MH  - Recombinant Fusion Proteins/physiology
MH  - Regulatory Sequences, Nucleic Acid
MH  - Trans-Activators/genetics/*physiology
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1038/sj.onc.1202584 [doi]
PST - ppublish
SO  - Oncogene. 1999 Apr 8;18(14):2311-21. doi: 10.1038/sj.onc.1202584.