PMID- 10327049
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20181201
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 14
DP  - 1999 Apr 8
TI  - Paired immunoglobulin-like receptor B (PIR-B) inhibits BCR-induced activation of 
      Syk and Btk by SHP-1.
PG  - 2291-7
AB  - Coligation of paired immunoglobulin-like receptor B (PIR-B) with B cell antigen
      receptor (BCR) blocks antigen-induced B cell activation. This inhibition is
      mediated in part by recruitment of SHP-1 and SHP-2 to the phosphorylated ITIMs in
      the cytoplasmic domain of PIR-B; however the molecular target(s) of these
      phosphatases remain elusive. Here we show that PIR-B ligation inhibits the
      BCR-induced tyrosine phosphorylation of Igalpha/Igbeta, Syk, Btk and
      phospholipase C (PLC)-gamma2. Overexpression of a catalytically inactive form of 
      SHP-1 prevents the PIR-B-mediated inhibition of tyrosine phosphorylation of Syk, 
      Btk, and PLC-gamma2. Dephosphorylation of Syk and Btk mediated by SHP-1 leads to 
      a decrease of their kinase activity, which in turn inhibits tyrosine
      phosphorylation of PLC-gamma2. Furthermore, we define a requirement for Lyn in
      mediating tyrosine phosphorylation of PIR-B. Based on these results, we propose a
      model of PIR-B-mediated inhibitory signaling in which coligation of PIR-B and BCR
      results in phosphorylation of ITIMs by Lyn, subsequent recruitment of SHP-1, and 
      a resulting inhibition of the BCR-induced inositol 1,4,5-trisphosphate generation
      by dephosphorylation of Syk and Btk.
FAU - Maeda, A
AU  - Maeda A
AD  - Department of Molecular Genetics, Institute for Liver Research, Kansai Medical
      University, Moriguchi, Japan.
FAU - Scharenberg, A M
AU  - Scharenberg AM
FAU - Tsukada, S
AU  - Tsukada S
FAU - Bolen, J B
AU  - Bolen JB
FAU - Kinet, J P
AU  - Kinet JP
FAU - Kurosaki, T
AU  - Kurosaki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Enzyme Precursors)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Pirb protein, mouse)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (inositol 3,4,5-trisphosphate)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Agammaglobulinaemia Tyrosine Kinase)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpn11 protein, mouse)
RN  - EC 3.1.3.48 (Ptpn6 protein, mouse)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Agammaglobulinaemia Tyrosine Kinase
MH  - Animals
MH  - Calcium Signaling/physiology
MH  - Cell Line
MH  - Enzyme Activation
MH  - Enzyme Precursors/*metabolism
MH  - Inositol Phosphates/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Isoenzymes/antagonists & inhibitors
MH  - Mice
MH  - Models, Biological
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational/*physiology
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/*physiology
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Antigen, B-Cell/*immunology
MH  - Receptors, IgG/genetics
MH  - Receptors, Immunologic/genetics/*physiology
MH  - Recombinant Fusion Proteins/physiology
MH  - Syk Kinase
MH  - Transfection
MH  - Type C Phospholipases/antagonists & inhibitors
MH  - src-Family Kinases/metabolism
EDAT- 1999/05/18 00:00
MHDA- 1999/05/18 00:01
CRDT- 1999/05/18 00:00
PHST- 1999/05/18 00:00 [pubmed]
PHST- 1999/05/18 00:01 [medline]
PHST- 1999/05/18 00:00 [entrez]
AID - 10.1038/sj.onc.1202552 [doi]
PST - ppublish
SO  - Oncogene. 1999 Apr 8;18(14):2291-7. doi: 10.1038/sj.onc.1202552.