PMID- 10325425
OWN - NLM
STAT- MEDLINE
DCOM- 19990804
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 11
DP  - 1999 Jun 1
TI  - A system for rapid generation of coat color-tagged knockouts and defined
      chromosomal rearrangements in mice.
PG  - 2354-60
AB  - Gene targeting in mouse embryonic stem (ES) cells can be used to generate single 
      gene mutations or defined multi-megabase chromosomal rearrangements when applied 
      with the Cre- loxP recombination system. While single knockouts are essential for
      uncovering functions of cloned genes, chromosomal rearrangements are great
      genetic tools for mapping, mutagenesis screens and functional genomics. The
      conventional approach to generate mice with targeted alterations of the genome
      requires extensive molecular cloning to build targeting vectors and DNA-based
      genotyping for stock maintenance. Here we describe the design and construction of
      a two-library system to facilitate high throughput gene targeting and
      chromo-somal engineering. The unique feature of these libraries is that once a
      clone is isolated, it is essentially ready to be used for insertional targeting
      in ES cells. The two libraries each bear a complementary set of genetic markers
      tailored so that the vector can be used for Cre- loxP -based chromosome
      engineering as well as single knockouts. By incorporating mouse coat color
      markers into the vectors, we illustrate a widely applicable method for stock
      maintenance of ES cell-derived mice with single gene knockouts or more extensive 
      chromosomal rearrangements.
FAU - Zheng, B
AU  - Zheng B
AD  - Department of Molecular and Human Genetics and Howard Hughes Medical Institute,
      Baylor College of Medicine,1 Baylor Plaza, Houston, TX 77030, USA.
FAU - Mills, A A
AU  - Mills AA
FAU - Bradley, A
AU  - Bradley A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Agouti Signaling Protein)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Proteins)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Agouti Signaling Protein
MH  - Animals
MH  - *Gene Rearrangement
MH  - Gene Targeting/*methods
MH  - Hair Color/*genetics
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Mice, Knockout
MH  - Monophenol Monooxygenase/*genetics
MH  - Proteins/*genetics
MH  - Time Factors
PMC - PMC148802
EDAT- 1999/05/15 00:00
MHDA- 1999/05/15 00:01
CRDT- 1999/05/15 00:00
PHST- 1999/05/15 00:00 [pubmed]
PHST- 1999/05/15 00:01 [medline]
PHST- 1999/05/15 00:00 [entrez]
AID - gkc357 [pii]
AID - 10.1093/nar/27.11.2354 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Jun 1;27(11):2354-60. doi: 10.1093/nar/27.11.2354.