PMID- 10325416
OWN - NLM
STAT- MEDLINE
DCOM- 19990804
LR  - 20211203
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 11
DP  - 1999 Jun 1
TI  - The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression
      in normal tissues and overexpression in tumors.
PG  - 2291-8
AB  - DNA methylation in mammals is required for embryonic development, X chromosome
      inactivation and imprinting. Previous studies have shown that methylation
      patterns become abnormal in malignant cells and may contribute to tumorigenesis
      by improper de novo methylation and silencing of the promoters for
      growth-regulatory genes. RNA and protein levels of the DNA methyltransferase
      DNMT1 have been shown to be elevated in tumors, however murine stem cells lacking
      Dnmt1 are still able to de novo methylate viral DNA. The recent cloning of a new 
      family of DNA methyltransferases (Dnmt3a and Dnmt3b) in mouse which methylate
      hemimethylated and unmethylated templates with equal efficiencies make them
      candidates for the long sought de novo methyltransferases. We have investigated
      the expression of human DNMT1, 3a and 3b and found widespread, coordinate
      expression of all three transcripts in most normal tissues. Chromosomal mapping
      placed DNMT3a on chromosome 2p23 and DNMT3b on chromosome 20q11.2. Significant
      overexpression of DNMT3b was seen in tumors while DNMT1 and DNMT3a were only
      modestly over-expressed and with lower frequency. Lastly, several novel
      alternatively spliced forms of DNMT3b, which may have altered enzymatic activity,
      were found to be expressed in a tissue-specific manner.
FAU - Robertson, K D
AU  - Robertson KD
AD  - University of Southern California, Norris Comprehensive Cancer Center, MS 83,
      1441 Eastlake Avenue, Los Angeles, CA 90033, USA.
FAU - Uzvolgyi, E
AU  - Uzvolgyi E
FAU - Liang, G
AU  - Liang G
FAU - Talmadge, C
AU  - Talmadge C
FAU - Sumegi, J
AU  - Sumegi J
FAU - Gonzales, F A
AU  - Gonzales FA
FAU - Jones, P A
AU  - Jones PA
LA  - eng
SI  - GENBANK/AF129267
SI  - GENBANK/AF129268
SI  - GENBANK/AF129269
GR  - R35 CA 49758/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Complementary)
RN  - 0 (DNMT3A protein, human)
RN  - 0 (Dnmt3a protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA Methyltransferase 3A)
RN  - EC 2.1.1.37 (DNA methyltransferase 3B)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2
MH  - Chromosomes, Human, Pair 20
MH  - Colonic Neoplasms/*enzymology/genetics
MH  - DNA (Cytosine-5-)-Methyltransferases/*genetics
MH  - DNA Methyltransferase 3A
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Humans
MH  - Kidney Neoplasms/*enzymology/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Pancreatic Neoplasms/*enzymology/genetics
MH  - RNA, Messenger
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Urinary Bladder Neoplasms/*enzymology/genetics
PMC - PMC148793
EDAT- 1999/05/15 00:00
MHDA- 1999/05/15 00:01
CRDT- 1999/05/15 00:00
PHST- 1999/05/15 00:00 [pubmed]
PHST- 1999/05/15 00:01 [medline]
PHST- 1999/05/15 00:00 [entrez]
AID - gkc377 [pii]
AID - 10.1093/nar/27.11.2291 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Jun 1;27(11):2291-8. doi: 10.1093/nar/27.11.2291.