PMID- 10325410
OWN - NLM
STAT- MEDLINE
DCOM- 19990804
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 11
DP  - 1999 Jun 1
TI  - Interaction of influenza virus NS1 protein and the human homologue of Staufen in 
      vivo and in vitro.
PG  - 2241-7
AB  - A screening for human proteins capable of interacting with influenza virus NS1
      has been carried out using the two-hybrid genetic trap in yeast. A cDNA
      corresponding to the human homologue of Drosophila melanogaster Staufen protein
      (hStaufen) was isolated that fulfilled all genetic controls of the two-hybrid
      protocol. Using a hStaufen cDNA isolated from a lambda human library, the
      interaction of hStaufen and NS1 proteins was characterised in vivo and in vitro. 
      Co-transfection of NS1 cDNA and a partial cDNA of hStaufen led to the
      relocalisation of recombinant hStaufen protein from its normal accumulation site 
      in the cytoplasm to the nuclear location of NS1 protein. NS1 and hStaufen
      proteins could be co-immunoprecipitated from extracts of co-transfected cells and
      from mixtures of extracts containing either protein, as well as from extracts of 
      influenza virus-infected cells. Furthermore, both proteins co-localised in the
      ribosomal and polysomal fractions of influenza virus-infected cells. The
      interaction was also detected in pull-down experiments using a resin containing
      purified hStaufen and NS1 protein translated in vitro. Deletion mapping of the
      NS1 gene indicated that a mutant protein containing the N-terminal 81 amino acids
      is unable to interact with hStaufen, in spite of retaining full RNA-binding
      capacity. These results are discussed in relation to the possible mechanisms of
      action of hStaufen and its relevance for influenza virus infection.
FAU - Falcon, A M
AU  - Falcon AM
AD  - Centro Nacional de Biotecnologia (CSIC), Campus de Cantoblanco, 28049 Madrid,
      Spain.
FAU - Fortes, P
AU  - Fortes P
FAU - Marion, R M
AU  - Marion RM
FAU - Beloso, A
AU  - Beloso A
FAU - Ortin, J
AU  - Ortin J
LA  - eng
SI  - GENBANK/AJ132258
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (INS1 protein, influenza virus)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (STAU1 protein, human)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (staufen protein, mammalian)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Polyribosomes
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Viral Nonstructural Proteins/genetics/*metabolism
PMC - PMC148787
EDAT- 1999/05/15 00:00
MHDA- 1999/05/15 00:01
CRDT- 1999/05/15 00:00
PHST- 1999/05/15 00:00 [pubmed]
PHST- 1999/05/15 00:01 [medline]
PHST- 1999/05/15 00:00 [entrez]
AID - gkc369 [pii]
AID - 10.1093/nar/27.11.2241 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Jun 1;27(11):2241-7. doi: 10.1093/nar/27.11.2241.