PMID- 10325225
OWN - NLM
STAT- MEDLINE
DCOM- 19990608
LR  - 20191210
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 284
IP  - 5417
DP  - 1999 May 14
TI  - Interaction of RAFT1 with gephyrin required for rapamycin-sensitive signaling.
PG  - 1161-4
AB  - RAFT1 (rapamycin and FKBP12 target 1; also called FRAP or mTOR) is a member of
      the ATM (ataxia telangiectasia mutated)-related family of proteins and functions 
      as the in vivo mediator of the effects of the immunosuppressant rapamycin and as 
      an important regulator of messenger RNA translation. In mammalian cells RAFT1
      interacted with gephyrin, a widely expressed protein necessary for the clustering
      of glycine receptors at the cell membrane of neurons. RAFT1 mutants that could
      not associate with gephyrin failed to signal to downstream molecules, including
      the p70 ribosomal S6 kinase and the eIF-4E binding protein, 4E-BP1. The
      interaction with gephyrin ascribes a function to the large amino-terminal region 
      of an ATM-related protein and reveals a role in signal transduction for the
      clustering protein gephyrin.
FAU - Sabatini, D M
AU  - Sabatini DM
AD  - The Johns Hopkins University School of Medicine, Department of Neuroscience, 725 
      North Wolfe Street, Baltimore, MD 21205, USA.
FAU - Barrow, R K
AU  - Barrow RK
FAU - Blackshaw, S
AU  - Blackshaw S
FAU - Burnett, P E
AU  - Burnett PE
FAU - Lai, M M
AU  - Lai MM
FAU - Field, M E
AU  - Field ME
FAU - Bahr, B A
AU  - Bahr BA
FAU - Kirsch, J
AU  - Kirsch J
FAU - Betz, H
AU  - Betz H
FAU - Snyder, S H
AU  - Snyder SH
LA  - eng
GR  - DA-00074/DA/NIDA NIH HHS/United States
GR  - DA-00266/DA/NIDA NIH HHS/United States
GR  - GM-07309/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Glycine)
RN  - 0 (Repressor Proteins)
RN  - 0 (gephyrin)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Cytoplasm/metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphoproteins/*metabolism
MH  - Phosphorylation
MH  - *Phosphotransferases (Alcohol Group Acceptor)
MH  - Rats
MH  - Receptors, Glycine/metabolism
MH  - Repressor Proteins/metabolism
MH  - Ribosomal Protein S6 Kinases/*metabolism
MH  - *Signal Transduction
MH  - Sirolimus/*pharmacology
MH  - TOR Serine-Threonine Kinases
EDAT- 1999/05/15 00:00
MHDA- 1999/05/15 00:01
CRDT- 1999/05/15 00:00
PHST- 1999/05/15 00:00 [pubmed]
PHST- 1999/05/15 00:01 [medline]
PHST- 1999/05/15 00:00 [entrez]
AID - 10.1126/science.284.5417.1161 [doi]
PST - ppublish
SO  - Science. 1999 May 14;284(5417):1161-4. doi: 10.1126/science.284.5417.1161.