PMID- 10323864
OWN - NLM
STAT- MEDLINE
DCOM- 19990604
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 9
DP  - 1999 May 1
TI  - The human Tap protein is a nuclear mRNA export factor that contains novel
      RNA-binding and nucleocytoplasmic transport sequences.
PG  - 1126-39
AB  - The constitutive transport element (CTE) encoded by simian type D retroviruses
      directs unspliced viral RNAs into a nuclear export pathway that is congruent with
      the pathway used by cellular mRNAs. Here, we show that quail cells are refractory
      to CTE function but become highly permissive upon expression of the human Tap
      protein, a candidate CTE cofactor. Tap contains a novel sequence-specific RNA
      binding domain that is sufficient for CTE binding but inadequate to support CTE
      function. Using microinjection assays, we have defined two NLSs and one NES in
      Tap. Mutational inactivation of the Tap NES, which lies outside the RNA-binding
      domain, not only blocks Tap function but also generates dominant-negative forms
      of Tap. Whereas replacement of the Tap NES with the well-defined Rev NES rescues 
      the ability of Tap to support CTE function, this substitution also confers
      sensitivity to agents that block the activity of Crm1, the Rev NES cofactor.
      Together, these data validate Tap as the first human sequence-specific nuclear
      mRNA export factor and identify a novel type of NES that can support nuclear mRNA
      export but does not act via Crm1.
FAU - Kang, Y
AU  - Kang Y
AD  - Department of Genetics, Duke University Medical Center, Durham, North Carolina
      27710, USA.
FAU - Cullen, B R
AU  - Cullen BR
LA  - eng
SI  - GENBANK/AF112880
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (RNA, Messenger)
RN  - 0 (TAP1 protein, human)
SB  - IM
SB  - X
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Biological Transport, Active
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mason-Pfizer monkey virus/genetics
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nucleic Acid Conformation
MH  - Quail
MH  - RNA, Messenger/chemistry/genetics/*metabolism
PMC - PMC316956
EDAT- 1999/05/14 00:00
MHDA- 1999/05/14 00:01
CRDT- 1999/05/14 00:00
PHST- 1999/05/14 00:00 [pubmed]
PHST- 1999/05/14 00:01 [medline]
PHST- 1999/05/14 00:00 [entrez]
AID - 10.1101/gad.13.9.1126 [doi]
PST - ppublish
SO  - Genes Dev. 1999 May 1;13(9):1126-39. doi: 10.1101/gad.13.9.1126.